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Optofluidic Lab-On-Chips for High Throughput 3D Imaging of Cells and Tissues
Federico Sala1,2, Petra Paiè1, Roberto Memeo2, Roberto Osellame1,2, Andrea Farina1, Andrea Bassi2, Francesca Bragheri1
1Istituto di Fotonica e Nanotecnologie - Consiglio Nazionale delle Ricerche (IFN-CNR),, Italy; 2Dipartimento di Fisica - Politecnico di Milano
Optofludic lab-on-chips are compact microsystems that allow the manipulation, the analysis and the imaging of cells with high throughput even at the single cell level. The optofluidic microscopes on chip here presented are fabricated by femtosecond laser micromachining followed by chemical etching. The technique is ideally suited for rapid prototyping and allows 3D geometries and perfect alignment between microfluidic and optical components networks. The devices have been validated on cancer cell lines by imaging in three dimensions both clusters of cells growing on spheres and single cells.
Woei Ming Steve Lee, Xuefei He, Samantha Montague, Tao Xu, Elizabeth Gardiner
The Australian National University, Australia
The application of advanced optical imaging technology for platelet biology is in its infancy. In this talk, we shall introduce the potential impact of optical imaging in understanding the roles of platelets in thrombus formation and in hemostasis. We will summarize how techniques in optical imaging has enabled exploration of morphological, molecular and fluidic parameters that determine platelet adhesion, activation and consequent roles in thrombus formation. Finally, we discuss the convergence of multiple imaging modalities towards a complete understanding of platelet roles in thrombus formation.