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OF5: Optofluidics: Optofluidics in Single-Cell Screening
14:00 - 15:30
Session Chair: Christian Karnutsch, Hochschule Karlsruhe - Technik und Wirtschaft
Location:Room 22a, 2nd floor, ICM Light Engineering and Optofluidics
14:00 - 14:30 Invited
In-flow tomographic phase microscopy for single cell analysis
Francesco Merola, Pasquale Memmolo, Lisa Miccio, Martina Mugnano, Pietro Ferraro
CNR - Institute of Applied Sciences and Intelligent Systems, Italy
Tomographic phase microscopy is presented for 3D reconstructions of live cells flowing in microfluidic channel. Red blood cells, diatom algae and tumor cells are analyzed for diagnostic purposes. The technique allows us to investigate each single cell passing within the field of view of the camera and to analyze hundreds of cells per second, depending on the flux speed and the camera frame rate. Moreover, it can be extended to almost every class of cell.
14:30 - 14:45
3D particle focusing by tightly curving microfluidic loops
Petra Paiè1, Francesca Bragheri1, Dino Di Carlo2, Roberto Osellame1
1Istituto di Fotonica e Nanotecnologie (IFN)-CNR, Piazza Leonardo da Vinci 32, 20133 Milan, Italy; 2Department of Bioengineering, University of California, Los Angeles CA, 90095, USA
In this work we present a compact microfluidic device for high throughput particle focusing, ideal for on chip flow cytometer applications. The device, fabricated by femtosecond laser irradiation followed by chemical etching, presents an innovative 3D layout comprising a sequence of vertical loops and straight channels.
14:45 - 15:00
The design and realization of optical instrumentation for monitoring microorganisms
Ota Samek, Mojmír Šerý, Tadeáš Maňka, Zdeněk Pilát, Silvie Bernatová, Jan Ježek, Martin Šiler, Petr Jákl, Pavel Zemánek
Institute of Scientific Instruments of the Academy of Sciences of the Czech Republic
The significance of the research lies in the development of specialized instrumentation and methodology to characterize microorganisms in order to optimize cultivation process for biotechnological applications
15:00 - 15:30 Invited
All-optical laser-scanning optofluidic imaging for high-throughput single cell analysis
Kevin K. Tsia
The University of Hong Kong, Hong Kong S.A.R. (China)