Conference Agenda

Overview and details of the sessions of this conference. Please select a date or location to show only sessions at that day or location. Please select a single session for detailed view (with abstracts and downloads if available).

 
Session Overview
Date: Monday, 18/Mar/2019
10:00am
-
10:30am
WE: Welcome Session
Location: HS 14
Chair: Michael W Pfaffl, Technical University of Munich, Germany
Chair: Stephen Andrew Bustin, Anglia Ruskin University, United Kingdom
10:30am
-
12:30pm
NGB: Next Generation Biomarkers
Location: HS 14
Chair: Michael W Pfaffl, Technical University of Munich, Germany
 

Towards a Universal Diagnostic Test for Infection Using Rapid Nanopore Metagenomics

Justin O'Grady

Quadram Insitute Bioscience, United Kingdom



MRNA As A Forensic Target - A Bridge Too Far?

Stephen Andrew Bustin

Anglia Ruskin University, United Kingdom



HTPathwaySeq, a Novel Application for High-throughput RNA Sequencing Based Pathway Phenotyping

Pieter Mestdagh, Tom Maes, Manuel Luypaert, Nele Nys, Gert Van Peer, Ariane De Ganck, Carolina Fierro, Jan Hellemans, Jo Vandesompele

Biogazelle, Belgium



Expression Data Integration: Advancing Immuno-Oncology Target Discovery

Frank Staubli, Jana Sponarova, Stefan Bleuler, Philip Zimmermann

Nebion AG, Switzerland

12:30pm
-
2:00pm
LU1: Lunch Monday
Location: Mensa
2:00pm
-
6:00pm
SC&MG: Single-Cell & Micro-Genomics
Location: HS 14
Chair: Mikael Kubista, TATAA Biocenter AB, Sweden
Chair: David Ruff, Mission Bio, Inc., United States of America
 

Spatial Transcriptomics - Bridging Histology and RNA Sequencing

Michaela Asp

KTH Royal Institute of Technology,Science for Life Laboratory,, Sweden



Spatial Analysis Of Transcriptome And Proteome During Early Development

Radek Sindelka, Pavel Abaffy, Ravindra Naraine, Mikael Kubista

Institute of Biotechnology, Czech Republic



High-throughput Single-cell Targeted DNA Sequencing from Tumor and Metastatic Samples Reveal Spatial Resolution of Evolutionary Trajectory Routes to Clonal Propagation

David Ruff

Mission Bio, Inc., United States of America



Using Single-cell Transcriptomics To Decipher The Formation Of Blood Stem Cells.

Christophe Lancrin

EMBL, Italy



Single-cell Genomics – Are We There Yet?

Malte Paulsen, Diana Ordonez, Bianka Baying, Dinko Pavlinic, Jonathan Landry, Vladimir Benes

EMBL, Germany, Flow Cytometry Core Facility, Genomics Core Facility



Tailoring scRNA-seq to Meet the Challenges of Primary Cytotoxic T-cells

Kristiyan Kanev1, Patrick Roelli1,3, Ming Wu1, Christine Wurmser2, Mauro Delorenzi3, Michael Pfaffl1, Dietmar Zehn1

1: Division of Animal Physiology and Immunology, School of Life Sciences Weihenstephan, Technical University of Munich, 85354 Freising, Germany; 2: Division of Animal Breeding, School of Life Sciences Weihenstephan, Technical University of Munich, 85354 Freising, Germany; 3: BCF, Swiss Institute of Bioinformatics, University of Lausanne 1015 Lausanne, Switzerland



A Deep Dive Into Single Cell RNA Sequencing Data

Patrick Rölli1, Kristiyan Kanev1, Delorenzi Mauro2, Zehn Dietmar1

1: TUM, Germany; 2: SIB, Switzerland

NGS-DA: NGS & NGS Data Analysis
Location: HS 15
Chair: Jo Vandesompele, Ghent University & Biogazelle, Belgium
Chair: David Langenberger, ecSeq Bioinformatics GmbH, Germany
 

RNA Atlas: A Nucleotide Resolution Map Of The Human Transcriptome

Lucia Lorenzi1, Francisco Avila Cobos1, Hua-Sheng Chiu2, Robrecht Cannoodt1, Tine Goovaerts1, Thomas Birkballe Hansen3, Pieter-Jan Volders1, Steve Gross4, Tom Taghon1, Karim Vermaelen1, Ken Bracke1, Jeroen Galle1, Jorgen Kjems3, Tim De Meyer1, Gary Schroth4, Pavel Sumazin2, Jo Vandesompele1, Pieter Mestdagh1

1: Ghent University, Belgium; 2: Baylor College of Medicine, US; 3: Aarhus University, Denmark; 4: Illumina, US



Getting More out of RNA-Seq Data: Transcriptomic Analysis of Ischemic Stroke

Peter Androvic1,3, Lukas Valihrach1, Denisa Kirdajova2, Martin Valny2, Miroslava Anderova2, Mikael Kubista1

1: Institute of Biotechnology CAS, Czech Republic; 2: Institute of Experimental Medicine CAS, Czech Republic; 3: Faculty of Science, Palacky University Olomouc, Czech Republic



Liquid Biopsies For Personalized Medicine: The Omiterc Project

Francesca Salvianti, Irene Mancini, Lisa Simi, Stefania Gelmini, Mario Pazzagli, Pamela Pinzani

University of Florence, Italy



Tiled Amplicons Panels in NGS-Based Genetic Testing

David Langenberger

ecSeq Bioinformatics GmbH, Germany



PCR Based Target Enrichment For Variant Confirmation, Gene Panels And Multiplex PCR Sample Tracking In A Whole Exome Sequencing Workflow

Frauke Coppieters1,2, Thalia Van Laethem3, Matthias De Smet1, Paul Coucke1,3, Kathleen Claes1,3, Elfride De Baere1,3, Björn Menten1,3, Jo Vandesompele2,3, Steve Lefever2,3

1: Center for Medical Genetics Ghent, Ghent University Hospital, Ghent, Belgium; 2: pxlence bvba, Dendermonde, Belgium; 3: Center for Medical Genetics Ghent, Ghent University, Ghent, Belgium



Handling of Spurious Molecular Species Dictates the Outcome of High-throughput 16S rRNA Gene Amplicon Profiling.

Sandra Reitmeier

ZIEL Institute for Food and Health, Core Facility Microbiome/NGS, Technical University of Munich, Freising, Germany



QC Measurements for Predicting Downstream NGS Success with FFPE and Circulating Cell-Free DNA Plasma Samples

Christopher D'Jamoos

Promega Corporation, 2800 Woods Hollow Rd. Madison, WI

6:00pm
-
10:00pm
PO1: Monday Main Poster Session
Location: Lower Level
All posters will be displayed in parallel at all three poster sessions PO1, PO2 & PO3 => the poster viewing can be done from "Monday Evening Main Poster Session" till "Wednesday Lunch Poster Session"
 

Digital PCR To Quantify Evolution Of Mycobacterium Tuberculosis SNP Proportion During In Vitro Competitive Assay

Charlotte Genestet1, Cécile Jacot-des-Combes2, Elisabeth Hodille1,3, Fabiola Bastian2, Oana Dumitrescu1,3

1: Centre International de Recherche en Infectiologie, INSERM U1111, Université de Lyon, Lyon, France; 2: Plateforme DTAMB, CNRS, Université Lyon 1, Villeurbanne, France; 3: Hospices Civils de Lyon, Institut des Agents Infectieux, Laboratoire de bactériologie, Lyon, France



Development of a Strain-specific Droplet Digital PCR (ddPCR) for Detection and Quantification of a Probiotic Strain Bifidobacterium animalis (BAN) in Feed.

Sergi Raurich, Michaela Mohnl, Viviana Klose, Gerd Schatzmayr, Silvia Fibi-Smetana

BIOMIN Research Center, Technopark 1A, 3430 Tulln, Austria



Design of a Digital PCR Assay for the Simultaneous Quantification of 14 Genetically Modified Soybean Lines in a Single Reaction

Katharina Lührig, Maximilian Neugebauer, Florian Priller, Heike Ziebarth, Hans-Henno Dörries, Cordt Grönewald, Kornelia Berghof-Jäger

BIOTECON Diagnostics GmbH, Germany



A Rapid, Simple, High-throughput Compatible Approach to Generating CRISPR/Cas9 Knock-out Cell Lines

Meiye Wu, Steven Okino, Gerald Ui

Reagent R&D, Life Sciences Group, Bio-Rad Laboratories,



The Good, the Bad and the Ugly: Selective single cell isolation

Sandra Lubos1, Nils Körber1, Heide Marie Resch1, Iris Augustin2, Stefan Niehren1

1: MMI - Microscopic Single Cell Isolation, Germany; 2: University of Applied Sciences, Weihenstephan-Triesdorf, Germany



A SMARTer Solution To Stranded Single-Cell RNA-Seq

Matthieu Pesant1, Suvarna Gandlur2, Nathalie Bolduc2, Simon Lee2, Christopher Hardy2, Ankita Das2, Magnolia Bostick2, Andrew Farmer2

1: Takara Bio Europe, Saint-Germain-en-Laye, France; 2: Takara Bio USA, Inc., Mountain View, CA 94043, USA



TATAA Alu Repeat qPCR Assays – a Tool for Contamination Control and Quality Assessment in Single Cell and Cell-Free DNA Analysis

Alexandra Bergman, Andrei Herdean, Robert Sjöback, Mikael Kubista

TATAA Biocenter, Sweden



SureCellⓇ ATAC-Seq Library Prep Kit: A New Solution for Single-Cell ATAC-Seq using Bio-Rad’s Droplet Digital Technology

Ronald Lebofsky, Carolyn Reifsnyder

Bio-Rad Laboratories, Digital Biology Center, Pleasanton CA



Single-cell studies: Focus on reverse transcription

Daniel Žucha1, Peter Androvič1, Mikael Kubista2, Valihrach Lukáš1

1: Laboratory of Gene Expression, Institute of Biotechnology CAS, Czech Republic; 2: TATAA Biocenter, Sweden



High-througput, full-length, single-cell RNA sequencing

François-Xavier Sicot1, Kazuo Tori2, Magnolia Bostick2, Shally Wang2, Andrew Farmer2

1: Takara Bio Europe, Saint-Germain-en-Laye, France; 2: Takara Bio USA, Inc., Mountain View, CA 94043, USA



Clinicаl Аpplicаtion of Next Generаtion Sequencing Technology Methods

Elham El Darazi

USEK, Lebanon (Lebanese Republic)



Full-length 16S rRNA Sequencing Combined with the Illumina Barcode Structure Allows a Deeper Insight into Strains Present in Stool Samples

Isabel Abellan-Schneyder, Ilias Lagkouvardos, Klaus Neuhaus

Core Facility Microbiome/NGS, ZIEL - Institute for Food and Health,Technische Universität München, Germany



Simple Whole Genome Amplification Technique Developed on the Basis of SD DNA Polymerase

Andreas Kirsten, Konstantin Ignatov, Ferdinand Holzinger, Vladimir Kramarov, Sergey Kovalenko

Bioron, Germany



Tackling Contaminants In Skin Microbiome Research

Claudia Hülpüsch1, Thomas Nussbaumer1, Vera Schwierzeck1, Amedeo De Tomassi1, Matthias Reiger1, Claudia Traidl-Hoffmann1,2, Avidan Neumann1

1: Institute of Environmental Medicine, UNIKA-T, Augsburg, Germany; 2: ZIEL - Institute for Food & Health, Freising, Germany



Microsatellite Instability Analysis and NGS with Fragmented Sample Types

Christopher D'Jamoos, Samantha Lewis, Henk Honing, Brad Hook, Curtis Knox, Katie Oostdik

Promega Corporation, United States of America



RiboPOOL: An Affordable Custom/Ribosomal RNA Depletion Solution Against Any Species for RNA Sequencing

Catherine Goh1, Andrew Walsh1, Michaela Beitzinger1, Jonas Bertram1, Kristina Doering3, Iana Kim4, Stefan Kotschote2, Claus Kuhn4, Konrad Foerstner3, Michael Bonin1

1: siTOOLs Biotech GmbH; 2: IMGM Laboratories GmbH; 3: University of Würzburg; 4: University of Bayreuth



Sample Quality Control of Long Read Sequencing and Low Input Libraries

Kyle Luttgeharm1, Bettina Strauch2, Samina Kaufmann2

1: Agilent Technologies, USA; 2: Agilent Technologies, Germany



A Guide for Sample Processing to Determine Novel Mitochondrial Genomes by Next-Generation Sequencing

Sarah Viola Emser1, Martin Hofer1, Daniela Allmer1, Ingeborg Klymiuk2, Eva Millesi3, Ralf Steinborn1

1: University of Veterinary Medicine, Vienna, Austria; 2: Core Facility Molecular Biology, Center for Medical Research, Medical University Graz, Graz, Austria; 3: Department of Behavioural Biology, University of Vienna, Vienna, Austria



Real-time Genotyping PCR From Blood And Blood Plasma Samples

Moritz Welter1,2, Ramon Kranaster2

1: Chair of Organic Chemistry / Cellular Chemistry, University of Konstanz, Universitätsstraße 10, 78457 Konstanz, Germany; 2: myPOLS Biotec GmbH, Technologiezentrum Konstanz, Blarerstraße 56, 78462 Konstanz, Germany, www.mypols.de



Considerations and Quality Controls when Analyzing Cell-free DNA

Gustav Johansson1,2,3, Daniel Andersson1, Stefan Filges1, Junrui Li1, Helena Kristiansson2,6, Tobias Österlund2,6, Andreas Muth4, Tony E. Godfrey5, Anders Ståhlberg1,2,6

1: Sahlgrenska Cancer Center, Department of Pathology and Genetics, Institute of Biomedicine, Sahlgrenska Academy at University of Gothenburg, Medicinaregatan 1F, 413 90 Gothenburg, Sweden; 2: Wallenberg Centre for Molecular and Translational Medicine, University of Gothenburg, Gothenburg, Sweden.; 3: Respiratory Inflammation and Autoimmunity, IMED Biotech Unit, AstraZeneca, Gothenburg, Sweden.; 4: Department of Surgery, Institute of Clinical Sciences, Sahlgrenska Academy at the University of Gothenburg, Gothenburg, Sweden; 5: Department of Surgery, Boston University School of Medicine, 700 Albany Street, Boston, MA 02118, USA; 6: Department of Clinical Pathology and Genetics, Sahlgrenska University Hospital, 413 45 Gothenburg, Sweden.



Analysis Of Cell-free Tumor DNA Using SiMSen-Seq

Helena Kristiansson1,2, Daniel Andersson3, Tobias Österlund1,2, Stefan Filges3, Gustav Johansson2,3,6, Junrui Li3, Melita Kaltak3, Åsa Torinsson Naluai4,5, Anders Ståhlberg1,2,3

1: Department of Clinical Pathology and Genetics, Sahlgrenska University Hospital, 413 45 Gothenburg, Sweden.; 2: Wallenberg Centre for Molecular and Translational Medicine, University of Gothenburg, Gothenburg, Sweden.; 3: Sahlgrenska Cancer Center, Department of Laboratory Medicine, Sahlgrenska Academy at University of Gothenburg.; 4: Department of Laboratory Medicine, Biobank Core Facility, University of Gothenburg.; 5: Biobank West, Sahlgrenska University Hospital, Sweden.; 6: Respiratory Inflammation and Autoimmunity, IMED Biotech Unit, AstraZeneca, Gothenburg, Sweden.



Performance Evaluation of Novel Protocols for Small RNA Sequencing In Biofluids

Sarka Benesova1,2, Peter Androvic1,3, Mikael Kubista1,4, Lukas Valihrach1

1: Laboratory of Gene Expression, Institute of Biotechnology CAS, BIOCEV, Vestec, Czech Republic; 2: Faculty of Chemical Technology, University of Chemistry and Technology, Prague, Czech Republic; 3: Faculty of Science, Palacký University, Olomouc, Czech Republic; 4: TATAA Biocenter, Gothenburg, Sweden



Taqman Advanced Mirna Cdna Synthesis Kit To Simultaneously Study Expression Of Mirna And Mrna From Serum Samples

Astrid Ferlinz

Thermo Fisher Scientific, United Kingdom



New Developments in Nucleic Acid Sample Quality Control

Bettina Strauch1, Rainer Nitsche1, Christine Voigt2, Martin Judex1

1: Agilent Technologies, Germany; 2: Alacris Theranostics, Germany



Plasma cfRNA Profiling using the NanoString nCounter® Low RNA Input Assay

Alan Huang, Christoph Konig

NanoString, United States of America



Detection and Analysis of Free Circulating Long non-coding RNAs and mRNAs in Colorectal Cancer Pantients.

Pavel Ostasov, Pavel Pitule

Biomedical Center, Faculty of Medicine in Pilsen, Charles University, Czech Republic



Detecting Alzheimer’s Disease Risk Factors by qPCR Directly on Blood or Saliva Samples

Andrei Herdean, Mikael Kubista, Robert Sjöback

TATAA Biocenter, Sweden



Building High Quality NGS DNA Libraries from Ultra-small Fragments

Stephan Bauer, Robert Brazas, Scott Monsma, Svetlana Jasinovica, Brandon Converse, Michael Lades

LGC, Biosearch Technologies, United States of America



Liquid Profiling of Head and Neck Cancer: Circulating cell-free DNA in Plasma and Saliva for minimally invasive Cancer Monitoring

Romina Rösch1,2, Irina Kerle2, Markus Wirth3,6, Markus Nieberler4, Nicole Pfarr5, Carolin Mogler5, Silvia Thoene1,6, Ramona Secci1,6, Andreas Bietenbeck1, Florian Bassermann2,6, Jürgen Ruland1,6, Christof Winter1,6

1: Department of Clinical Chemistry and Pathobiochemistry, Klinikum rechts der Isar, Technische Universität München, Munich, Germany; 2: Department of Internal Medicine III - Hematooncology, Klinikum rechts der Isar, Technische Universität München, Munich, Germany; 3: Department of Otolaryngology, Klinikum rechts der Isar, Technische Universität München, Munich, Germany; 4: Department of Oral and Maxillofacial Surgery, Klinikum rechts der Isar, Technische Universität München, Munich, Germany; 5: Department of General Pathology and Pathologcial Anatomy, Technische Universität München, Munich, Germany; 6: German Cancer Consortium (DKTK), partner site Munich; and German Cancer Research Center (DKFZ), Heidelberg, Germany



Anesthesia and Its Impact on miRNA Profiles in Circulating Extracellular Vesicles During Cancer Surgery

Dominik Buschmann1, Florian Brandes2,3, Anja Lindemann3, Melanie Maerte2, Petra Ganschow4, Alexander Chouker2, Gustav Schelling2, Michael W. Pfaffl1, Marlene Reithmair3

1: Division of Animal Physiology and Immunology, Technical University of Munich, Freising, Germany; 2: Department of Anesthesiology, University Hospital, Ludwig-Maximilians-University Munich, Munich, Germany; 3: Institute of Human Genetics, University Hospital, Ludwig-Maximilians-University Munich, Munich, Germany; 4: Department of General, Visceral, and Transplant Surgery, Ludwig-Maximilians-University Munich, Munich, Germany



Sequence-specific release of EV-associated RNAs

Marie Mosbach, Christian Preußer, Lee-Hsueh Hung, Albrecht Bindereif

Justus Liebig University of Giessen, Institute of Biochemistry, Germany



Analysis of Total RNA Yield and Purity Using Different Extracellular Vesicle Isolation Methods

Rebekka Van Hoof1,2, Karen Hollanders1, Sarah Deville1,3, Patrick Wagner4, Jef Hooyberghs1,2, Inge Nelissen1

1: Sustainable Health, VITO (Flemish Institute for Technological Research), Mol, Belgium; 2: Theoretical Physics, Hasselt University, Diepenbeek, Belgium; 3: Biomedical Research Institute, Hasselt University, Diepenbeek, Belgium; 4: Laboratory for Soft Matter and Biophysics, University of Leuven, Leuven, Belgium



Urinary Extracellular Vesicles: Unveiling the Most Appropriate Purification Method with a View to RNA Sequencing and Biomarker Profiling

Veronika Mussack1, Georg Wittmann2, Michael W Pfaffl1

1: Animal Physiology and Immunology, TUM School of Life Sciences Weihenstephan, Freising, Germany; 2: Department for Transfusion Medicine, Cell therapeutics and Haemostaseology, University Hospital LMU, Munich, Germany



Validating Sensitive Workflows To Analyze RNAs in Tumor-derived Extracellular Vesicles in Serum and Urine Samples of NSCLC Patients

Vera Kloten1, Rita Lampignano1, Martin Neumann2, Nina Kessler3, Anna Babayan2, Klaus Pantel3, Thomas Krahn1, Thomas Schlange1

1: Bayer AG, Germany; 2: Qiagen, Germany; 3: Department of Tumor Biology, Germany



Serum-Free Media Supplements Carry MiRNAs That Co-Purify With Extracellular Vesicles

Martin Auber, Dominik Fröhlich, Krämer-Albers Eva-Maria

Johannes Gutenberg University of Mainz, Germany



Systematic Comparison of Extracellular Vesicles from Human Arterial and Venous Blood: Highly Identical microRNA Expression Indicates Equal Use for Biomarker Applications

Stefanie Hermann1, Dominik Buschmann1, Benedikt Kirchner1, Melanie Märte2, Florian Brandes2, Stefan Kotschote3, Michael Bonin3, Marlene Reithmair4, Gustav Schelling2, Michael W. Pfaffl1

1: Division of Animal Physiology and Immunology, School of Life Sciences Weihenstephan, Technical University of Munich, Germany; 2: Department of Anesthesiology, University Hospital, Ludwig-Maximilians-University Munich, Germany; 3: IMGM Laboratories GmbH, Planegg, Germany; 4: Institute of Human Genetics, University Hospital, Ludwig-Maximilians-University Munich, Germany



Grp78 Plays A Crucial Role In The Exosome-Promoted Survival Of Irradiated Squamous Head And Neck Cancer Cells

Michael Schneider, Lisa Mutschelknaus, Klaudia Winkler, Rosemarie Kell, Michael J. Atkinson, Simone Moertl

Helmholtz Zentrum München, Germany



Analysis Of DNA Content From Human Melanoma Cellline Derived Extracellular Vesicles

Daniela Marie Brodesser1,2,3, Ingrid Walter4, Waltraud Tschulenk4, Sabine Brandt3, Gottfried Brem2, Franz Trautinger5, Joerg P. Burgstaller1,2

1: Biotechnology in Animal Production, IFA Tulln, Austria; 2: Institute of Animal Breeding and Genetics, Vetmeduni Vienna; 3: Research Group Oncology, Equine Clinic, Vetmeduni Vienna; 4: Institute of Pathology and Forensic Medicine, Vetmeduni Vienna; 5: Department of Dermatology and Venereology, Karl Landsteiner University of Health Sciences



P3py: A Python Module For The Multi-array MicroRNA Detection Data Analysis And The simulation Of Biomarker Diffusion And Reaction

Bo Zeng, Christiane Geithe, Stefan Rödiger

BTU Cottbus Senftenberg, Germany



Phenovault: An Open-Access Resource For Analysing RNAi And CRISPR Screens

Catherine Goh, Andrew Walsh, Michaela Beitzinger, Stefan Hannus, Michael Hannus

siTOOLs Biotech, Germany



Optimal Use Of Statistical Methods To Validate Reference Gene Stability In Longitudinal Studies

Venkat Krishnan Sundaram1,2, Nirmal Kumar Sampathkumar1,2, Charbel Massaad1,2, Julien Grenier1,2

1: Université Paris Descartes, France; 2: INSERM U1124, Myelination and Nervous system pathologies - Group



A New Method to prepare DNA Libraries for NGS by using SD DNA Polymerase

Andreas Kirsten, Ferdinand Holzinger, Konstantin Ignatov, Vladimir Kramarov, Sergey Kovalenko

Bioron, Germany



BIOCEV GeneCore – More Than Just Gene Expression Profiling

Lucie Langerová, Filip Franko, Eva Rohlová, David Švec

Institute of Biotechnology CAS, Czech Republic



MLH1 Promoter Hypermethylation: Development and Validation of a Methylation-Sensitive High-Resolution Melting (MS-HRM) Assay for use in a Lynch Syndrome Pre-Screen Pathway

Diana Pelka1,2, Sasha Hansel1, Ben Poskitt1, David Moore1,3, Tomasz K Wojdacz4,5, Phil Bennett1, Gareth Gerrard1,2

1: Sarah Cannon Molecular Diagnostics, HCA Healthcare UK, London, UK; 2: Faculty of Medicine, Imperial College London, London, UK; 3: Department of Histopathology, UCLH NHS Foundation Trust, London, UK; 4: MethylDetect ApS, Aalborg, Denmark; 5: Pomeranian Medical University, Szczecin, Poland



Detection And Quantification Of MicroRNAs Using A Multiplex Microbead Assay To Accompany Quantitative PCR And Digital PCR Methods

Christiane Geithe1, Dirk Roggenbuck2, Katja Hanack3, Peter Schierack1, Stefan Rödiger1

1: BTU Cottbus-Senftenberg, Chair of Multiparameter Diagnostics, Senftenberg, Germany; 2: GA Generic Assays GmbH, Blankenfelde-Mahlow, Germany; 3: University of Potsdam, Institute of Biochemistry and Biology, Professorship of Immunotechnology, Potsdam-Golm, Germany



Developing a Customizable Panel of Real-time qPCR Assays on a Microfluidic Device for Respiratory Tract Pathogen Detection

Emmanuelle Lenotre

Thermo Fisher Scientific, United Kingdom



Methods to determine Limit of Detection and Limit of Quantification of a quantitative real-time PCR (qPCR).

Amin Forootan

Multid Analyses AB, Sweden



A Winter`s Week Tale in Sheffield Virology Department

Mehmet Onder Yavuz

Sheffield Teaching Hospitals NHS Trust, United Kingdom



Effects Of The Use Of Degenerate Bases In Primers On Quantitative PCR Measurements

Laure Barbier, Catherine Rousseau, Charles Chapus, Thomas Poyot

IRBA, France



Evaluation of DNA Fragmentation Methods for Implementation of cgMLST in Routine Analysis

Larissa Murr, Melanie Pavlovic, Ingrid Huber, Ulrich Busch, Patrick Guertler

Bavarian Health and Food Safety Authority, Veterinärstr. 2, 85764 Oberschleißheim, Germany



FASTFISH-ID™: ThermaGenix’s Rapid DNA Authentication of Any Species of Commercial Fish Using Bio-Molecular Systems’ MIC sPCR Cycler.

Jesus Aquiles Sanchez1, Amanda M. Naaum2, Marine Cusa3, Ian Goodhead3, Sarah Helyar2, Albert Wijngaard4, Christopher Elliot2, Stefano Mariani3, Lawrence Wangh1

1: Thermagenix, Inc,, Natick, Massachusetts, United States of America; 2: Institute for Global Food Security, Queen’s University Belfast, Belfast, United Kingdom; 3: School of Environment and Life Sciences, University of Salford, Salford, United Kingdom; 4: Bio Molecular Systems, Upper Coomera, QLD, Australia



Is The High-Throughput human OpenArray® System Useful For Profiling miRNAs In Melanoma Regression In A Swine Model?

Claudia Bevilacqua1, Fany Blanc1, Marie-Noelle Rossignol1, Guillaume Piton2, Sylvain Marthey1, Silvia Vincent-Naulleau2

1: INRA, France; 2: CEA, France



New Advances in Two-Tailed RT-qPCR

Eva Rohlova1,2, Mansi Maheta1,2, Peter Androvic1,3, Lukas Valihrach1, Mikael Kubista1,4

1: Laboratory of Gene Expression, Institute of Biotechnology CAS, BIOCEV, Vestec, Czech Republic; 2: Faculty of Science, Charles University, Prague, Czech Republic; 3: Faculty of Science, Palacký University, Olomouc, Czech Republic; 4: TATAA Biocenter, Gothenburg, Sweden



New Versatile Enzymes for High Performance Multiplex RNA Detection and Analysis

Ryan Heller, Suhman Chung, Kyle Dumas, Kasia Crissy, Patrick Barchard, David Schuster, Thomas Schoenfeld

QIAGEN Beverly, United States of America



Polymerase Strand Displacement Reaction with SD DNA Polymerase – New Alternative to PCR

Anke Fenn, Ferdinand Holzinger, Konstantin Ignatov, Vladimir Kramarov, Andreas Kirsten, Sergey Kovalenko

Bioron, Germany



QPCR, DPCR and NGS for The Detection of EGFR Mutations In Lung Cancer Patients

Francesca Salvianti, Gemma Sonnati, Filomena Costanza, Pamela Pinzani

University of Florence, Italy



SMARTer PicoPLEX Gold: A New Generation of Single Cell NGS Library with High Reproducibility, and Greatly Improved Coverage and Fidelity for Precision Medicine

Matthieu Pesant1, Fang Sun2, Datta Mellacheruvu2, Bayu Sisay2, John Langmore2, Graeme McLean2, Andrew Farmer2, Emmanuel Kamberov2

1: Takara Bio Europe, Saint-Germain-en-Laye, France; 2: Takara Bio USA, Inc., Mountain View, CA 94043, USA



Sample quality control of Cell-free DNA

Bettina Strauch, Eva Graf, Elisa Viering

Agilent Technologies, Germany



SiPOOL: Fast, Reliable Gene Silencing With Exceptional Target Specificity Using Optimally-Designed Complex siRNA Pools

Catherine Goh1, Andrew Walsh1, Michaela Beitzinger1, Jonas Bertram1, Stefan Hannus1, Gunter Meister2, Michael Hannus1

1: siTOOLs Biotech GmbH; 2: University of Regensburg



Small Circular RNAs With Antisense Function

Christina Pfafenrot, Albrecht Bindereif

Justus Liebig University of Giessen, Germany



Streamlined Single-tube Solutions for High Quality DNA Library Preparation

Yi Jing, Marissa Bolduc, David Bays, Shuhong Li, Eleanor Kolossovski, Brian Komorous, Hongbo Liu, David Shuster

Quantabio, United States of America



TATAA Biocenter - Your Full Range Commissioned Service Provider. Get Better Results Faster

Hanna Zoric, Jens Björkman, Mikael Kubista

TATAA Biocenter, Sweden



The Spectrum Compact CE System: Fragment Analysis with a Personal Capillary Electrophoresis Device

Christopher D'Jamoos1, Ayaka Okuno2, Doug Storts1, Robert Mclaren1, Cynthia Sprecher1, Michiru Fujiocha2, Jin Matsumura2, Isao Haraura2, Asami Terakado2, Noriuki Sumida2

1: Promega Corporation, Madison, WI 53711, USA; 2: Hitachi High-Technologies Corporation, 882 Ichige, Hitachinaka-shi, Ibaraki-Ken, 312-8504 Japan



The Synthetic DNA Fragment Use in the qPCR Diagnostics

Martina Grochová1,2, Karel Bílek1, Kateřina Rosenbergová1

1: National Institute for NBC Protection (SÚJCHBO, v.v.i.), Czech Republic; 2: Faculty of Sciences, University of South Bohemia in České Budějovice, Czech Republic



ThermaGenix’s Universal PCR Additives for Suppressing DNA and RNA Amplification Errors.

Jesus Aquiles Sanchez, John Rice, Kenneth Pierce, Lawrence Wangh

Thermagenix, Inc,, Natick, Masaschusetts, United States of America



Use of Single Control DNA to Internally Evaluate Performance of qPCR and dPCR Instruments

Dejan Stebih, Katja Stare, Marjana Camloh, Mojca Milavec

National Institute of Biology, Slovenia



MOL-PCR and xMAP Technology: Fast Multiplex Method with High Sensitivity

Jirina Markova, Petr Kralik

Veterinary Research Institute Brno, Czech Republic



Exteme One-Step RT-qPCR: Potential for point-of-care viral detection.

Jessica A. Houskeeper, Lauryn Narramore, Carl T. Wittwer

Univeristy of Utah, United States of America



QC Measurements for Predicting Downstream NGS Success with FFPE and Circulating Cell-Free DNA Plasma Samples

Christopher D'Jamoos, Doug Wieczorek, Spencer Hermanson, Curtis Knox, Jennifer Mook, Eric Vincent, Doug Horejsh, Trista Schagat, Doug Storts

Promega Corporation, 2800 Woods Hollow Rd. Madison, WI



Combination of Hormone and Transcriptome Analyses in the Study of Cold and Freezing Stress in Grass Lolium perenne

Sylva Prerostova1, Barbara Kramna1,2, Jan Simura3, Alena Gaudinova1, Vojtech Knirsch1, Ondrej Novak3, Radomira Vankova1

1: Institute of Experimental Botany, Czech Academy od Sciences, Czech Republic; 2: Faculty of Science, Charles University, Czech Republic; 3: Centre of the Region Haná for Biotechnological and Agricultural Research, Palacký University and Czech Academy of Sciences, Czech Republic



Differentiation Potential of NG2-glia Following Different Types of Brain Injuries

Lukas Valihrach1, Denisa Kirdajova2, Denisa Kolenicova2, Daniela Krocianova2, Jan Kriska2, Peter Androvic1,3, Daniel Zucha1,4, Eva Rohlova1,4, Mansi Maheta1,4, Miroslava Anderova2, Mikael Kubista1,5

1: Institute of Biotechnology, Czech Republic; 2: Institute of Experimental Medicine, Czech Republic; 3: Faculty of Science, Palacký University, Czech Republic; 4: Faculty of Science, Charles University, Czech Republic; 5: TATAA Biocenter, Sweden



Metrological Support for Molecular Diagnostics - A Case Study of HCMV and Its Resistance to Ganciclovir

Alexandra Bogožalec Košir1, Tašja Cvelbar2, Mojca Milavec1

1: National Institute of Biology, Department of Biotechnology and Systems Biology, Slovenia; 2: Biotechnical Faculty, Department of Biology, University of Ljubljana, Slovenia



Detection of Bacteria and Virus Involved Urinary Tract Infections and Profiling Their Antibiotic Resistance Using TaqMan Real-time PCR Assays

Pius Brzoska, Xiaoqing You

Thermo Fisher Scientific, United States of America



Immunomodulatory Properties of Adipose-Derived Stem Cells Treated with 5-Azacytydine and Resveratrol on Peripheral Blood Mononuclear Cells and Macrophages in Metabolic Syndrome Animals

Krzysztof Marycz1,2, Katarzyna Kornicka1, Agnieszka Smieszek1

1: Wrocław University of Environmental and Life Sciences, Poland; 2: Faculty of Veterinary Medicine, Equine Clinic-Equine Surgery, Justus-Liebig-University, 35392 Gießen, Germany



Elucidation of the Role of Tenacibaculum spp. in Atypical Winter-ulcer in Sea-farmed Atlantic Salmon in Norway

Bjørn Spilsberg, Karin Lagesen, Hanne Katrine Nilsen, Anne Berit Olsen, Duncan Colquhoun

Norwegian Veterinary Institute, Norway



Investigation Of Association Of Variant Rs17036314 Of PPARG Gene And Rs7923837 Of HHEX Gene In Diabetes Mellitus Type 2 Patients In Iran Population By Tetra-ARMS PCR And Sanger DNA Sequencing

Maryam Majzoobi

IAUPS, Iran, Islamic Republic of



Microvesicles Derived From Human Adipose-Derived Multipotent Stromal Cells Improves Retinal Functionality in Dogs With Retinal Degeneration.

Agnieszka Śmieszek1, Anna Pakuluk2,3, Natalia Kucharczyk2,3, Peter G.C. Bedford4, Krzysztof Marycz1,5

1: 3Department of Experimental Biology, The Faculty of Biology and Animal Science, University of Environmental and Life Sciences Wroclaw 50-375, Poland; 2: Przychodnia weterynaryjna Trzebnicka; 3: Przychodnia weterynaryjna Viva; 4: Professor Emeritus of Veterinary Ophthalmology, Royal Veterinary College, London, UK; and Ophthalmology Referrals, 25, Great North Road, Brookmans Park, Herts. AL9 6LB, UK; 5: Faculty of Veterinary Medicine, Equine Clinic-Equine Surgery, Justus-Liebig-University, 35392 Gießen, Germany



Strigolactone Mediated Age - And Organ-Specific Gene Expression In Arabidopsis thaliana

Barbara Kramna1,2, Sylva Prerostova1, Eva Kobzova1, Radomira Vaňkova1

1: Institute of Experimental Botany, Czech Acad. Sci.,; 2: Charles University in Prague, Faculty of Science, Department of Experimental Biology of Plants



Role of Nitric Oxide During Embryonic Wound Healing

Pavel Abaffy, Ravindra Naraine, Silvie Tomankova, Radek Sindelka

Laboratory of Gene Expression, Institute of Biotechnology, Czech Academy of Science, Czech Republic



Study Of An In Vitro System For Alzheimer’s Disease

Luo Wei2, Larry Wong1, Deanna Woo2, Matthew Hammond2, Laura Moriarty2, Elisabeth Jordan Dreskin2

1: Bio-Rad Laboratories, Hercules, CA, USA; 2: Bio-Rad Laboratories, Mississauga, ON, Canada



The Expression of Prostaglandins during different Physiological stages in the Bovine Corpus Luteum

Bajram Berisha1,2, Dieter Schams2, Daniela Rodler3, Fred Sinowatz3, Michael Pfaffl2

1: University of Prishtina, Kosovo; 2: Animal Physiology & Immunology Weihenstephan, Technical University of Munich, Germany; 3: Department of Veterinary Sciences, Ludwig Maximilian University of Munich, Germany



Use of Universal ProbeLibrary Probes for Detection of Highly Pathogenic Biological Agents

Karel Bílek, Kateřina Rosenbergová, Martina Grochová, Hana Placáková, Oldřich Kubíček, Jiřina Procházková

National Institute for NBC Protection (SÚJCHBO, v.v.i.), Czech Republic



Using In Vitro Transcribed MRNA For Gene Therapy Targeting Viral Infections

Andreas Oswald, Ulrike Protzer

Institut für Virologie, Technische Universität München, Germany



Whole Genome Crispr Screening to Identify Potential SERD Molecule Resistance Mechanisms

Christophe Marcireau, Karine Berthelot, Alice Williard, Hamida Fournet, Delphine Debono, Gilbert Thill, Helene Erasimus, Dorine Chassin, Christophe Lanneau, Veeranagouda Yaligara, Cecile Orsini, Michel Didier, Vincent Mikol, Monsif Bouaboula, Laurent Debussche

Sanofi, France

ENJOY: Welcome Reception
Location: Lower & Upper Level

Date: Tuesday, 19/Mar/2019
8:30am
-
12:30pm
dPCR: digital PCR
Location: HS 14
Chair: Jim Huggett, University of Surrey, United Kingdom
Chair: Afif Abdel Nour, Universite Saint Esprit de Kaslik, Lebanon (Lebanese Republic)
 

Biometrology, What Is It And What Could It Mean For Biomolecular Research?

Jim Huggett

University of Surrey, United Kingdom



Molecular Characterization By ddPCR Of In Vitro Differentiated Oocyte-like Cells From Oogonial Stem Cells

Erica Silvestris, Paola Cafforio, Stella D'Oronzo, Claudia Felici, Francesco Silvestris, Giuseppe Loverro

University of Bari Aldo Moro, Italy



Higher Order Multiplexing Using Digital PCR for CRISPR Gene Edit Validation

Alexandra Sarah Whale

LGC, United Kingdom



Serial Flow Digital Droplet PCR, a New Paradigm!

Chris Perkins

Dropworks, Inc., United States of America



Eight Years Later This Is What Digital PCR Offered To Science

Afif Abdel Nour

The Holy Spirit University of Kaslik, Lebanon



Highly Multiplexed Detection of GMOs in Food Samples by Crystal Digital PCR

Katharina Lührig1, Caroline Charky2, Maximilian Neugebauer1, Heike Ziebarth1, Kornelia Berghof-Jäger1

1: BIOTECON Diagnostics GmbH, Germany; 2: Stilla Technologies, France



Resolving Translational Oncology Research Challenges with NanoString Direct Digital Gene Expression Analysis.

Christoph König

Nanostring Technologies, United Kingdom

LB: Liquid Biopsy
Location: HS 15
Chair: Jo Vandesompele, Ghent University & Biogazelle, Belgium
Chair: Michael W Pfaffl, Technical University of Munich, Germany
 

Cell-Free Tumor DNA Analysis In Liquid Biopsy Using Ultrasensitive Sequencing

Anders Ståhlberg1,2

1: University of Gothenburg, Sweden; 2: Sahlgrenska University Hospital, Sweden



Clinical Application of Liquid Profiling for Precision Medicine

Stefan Holdenrieder

German Heart Center Munich, Germany



Exploiting RNA in Liquid Biopsies for Precision Medicine Purposes

Jo Vandesompele1,2

1: Ghent University, Belgium; 2: Biogazelle, Belgium



Liquid Biopsy -- Exosomal microRNA Biomarker Signatures in Clinical Diagnostics.

Michael W Pfaffl

Division of Animal Physiology & Immunology, School of Life Sciences Weihenstephan, Technical University of Munich, Germany



New Developments in Sample Quality Control - Focus on Cell-free DNA

Bettina Strauch, Elisa Viering

Agilent Technologies, Germany



Exosome-derived Epigenetic Biomarkers for Saliva Diagnostics

Ulrike Kegler1, Manuela Hofner1, Anja Buhmann1, Helene Scharkosi1, Walter Pulverer1, Michael Leutner2, Klemens Vierlinger1, Alexandra Kautzky-Willer2, Christa Nöhammer1

1: AIT Austrian Institute of Technology GmbH, Molecular Diagnostics, Vienna, Austria; 2: Medical University Vienna, Division for Endocrinology and Metabolism, Vienna, Austria



Sensitive, Easy And Fast Monitoring Of Treatment Efficiency, Resistance Development And Disease Progression Using Open Platform QPCR On Liquid Biopsies From Cancer Patients

Ulf Bech Christensen, Rasmus Koefoed Petersen

PentaBase, Denmark

 
12:30pm
-
2:00pm
Journal: BDQ Editors Meeting
Location: HS 15
Chair: Jim Huggett, University of Surrey, United Kingdom
Chair: Justin O'Grady, Quadram Insitute Bioscience, United Kingdom
PO2: Tuesday Lunch Poster Session
Location: Lower Level
All posters will be displayed in parallel at all three poster sessions PO1, PO2 & PO3 => the poster viewing can be done from "Monday Evening Main Poster Session" till "Wednesday Lunch Poster Session"
LU2: Lunch Tuesday
Location: Mensa
2:00pm
-
6:00pm
AMDx1: Advanced Molecular Diagnostics 1
Location: HS 14
Chair: Carl T Wittwer, University of Utah, United States of America
Chair: Mikael Kubista, TATAA Biocenter AB, Sweden
 

Rapid qPCR and the Future of Molecular Diagnostics

Carl T Wittwer

University of Utah, United States of America



Impact of Endogenous and Exogenous Alterations of Glucocorticoids on the Derangement of Clock-related Genes in Peripheral Mononuclear Cells

Mary Anna Venneri

Sapienza University of Rome, Italy



SD DNA Polymerase: A New Tool for Variety of Molecular Biology Applications

Konstantin Ignatov, Andreas Kirsten, Vladimir Kramarov, Ferdinand Holzinger, Sergey Kovalenko

Bioron, Germany



Methylation Sensitive High Resolution Melting; A Sensitive And Specific Method For High-Throughput Assessment Of Methylation.

Massimo Santoro

IRCCS Fondazione Don Carlo Gnocchi, Italy



Two-Tailed Quantitative PCR for Precison Diagnostics

Mikael Kubista1,2, Robert Sjöback1, Andrei Herdean1, Peter Androvic2, Lukas Valihrach2

1: TATAA Biocenter AB, Sweden; 2: Institute of Biotechnology, Czech Academy of Sciences



Precision Liquid Biopsy Based Nucleic Acid Based Molecular Diagnostics Powered by Xenonucleic Acids

Michael Joseph Powell

DiaCarta, Inc., United States of America



Multiplex xMAP Technology for a Complex Detection of Pathogenic Agents Significant for the Protection of Human and Animal Health

Pavlina Jelinkova, Petr Kralik

Veterinary research institute/Brno, Czech Republic

ISEV: ISEV Satellite Session
Location: HS 15
Chair: Kenneth Witwer, The Johns Hopkins University School of Medicine, United States of America
Chair: Hidetoshi Tahara, Hiroshima University, Japan
 

Mesenchymal Stem Cell-derived Extracellular Vesicles: a Potential New Tool in Regenerative Medicine

Bernd Giebel

Institute for Transfusion Medicine, Germany



Standardization in EV and exRNA Research: MISEV and Beyond

Kenneth Witwer

The Johns Hopkins University School of Medicine, United States of America



Circular RNAs In EVs - A Novel Class Of Released Non-coding RNAs

Christian Preußer

Justus Liebig University of Giessen, Institute of Biochemistry



Characterization of Extracellular vesicles (EVs) Derived ExRNA in Cancer Using NGS

Yukie Nishiyama1, Makoto Tahara2, Hidetoshi Tahara1

1: Department of Cellular and Molecular Biology,Institute of Biomedical & Health Sciences, Hiroshima university, Japan; 2: Department of Head and Neck Medical Oncology, National Cancer Center Hospital East, Kashiwa, Japan.



Distribution Of Non-coding RNA Types Over EV And Other RNA Carriers In Plasma

Esther Nolte-'t Hoen, Tom Driedonks, Susanne van der Grein

Utrecht University, The Netherlands



Normalizing Urinary Extracellular Messenger RNA Biomarkers: Theoretical Considerations and a Review of Experimental Findings

Pradeep Moon Gunasekaran1, J. Matthew Luther2, J. Brian Byrd1

1: University of Michigan Medical School, United States of America; 2: Vanderbilt University School of Medicine, United States of America



Serum-Free Media Supplements Carry MiRNAs That Co-Purify With Extracellular Vesicles

Martin Auber, Dominik Fröhlich, Krämer-Albers Eva-Maria

Johannes Gutenberg University of Mainz, Germany

 
7:00pm
-
11:59pm
DINE: Event Dinner @ Bräustüberl
Location: Bräustüberl

Date: Wednesday, 20/Mar/2019
9:00am
-
10:30am
MULTI1: Multi-Omics 1
Location: HS 14
Chair: Justin O'Grady, Quadram Insitute Bioscience, United Kingdom
 

The End of Medicine as we know it.

Harald H.H.W. Schmidt

Department of Pharmacology and Personalised Medicine, Faculty of Health, Medicine and Life Sciences, Maastricht University, The Netherlands



Precision Medicine beyond Cancer: Why We Need New Multi-Omics Driven Definitions for Health & Disease

Thomas Wilckens

InnVentis Ltd, Germany



Circulating miRNAs as Potential Biomarkers

Tim Kacprowski

Technical University of Munich, Germany

MIQE&QC: MIQE Guidelines & Standardisation & Quality Control
Location: HS 15
Chair: Andreas Untergasser, Heidelberg University, Germany
 

Quality Standards in quantitative PCR; Specification, Validation, Controls and Standards

Peter Rossmanith

Vetmeduni Vienna, Austria



Directed Evolution of Enzymes for Streamlined and Reliable RT-qPCR and NGS Workflows

David Mark Schuster

Quantabio, United States of America



Multiplex Mediator Probe Real-Time PCR: Optimisation and Guideline Development through Systematic Characterisation of Label Free Mediator Probes and Fluorogenic Universal Reporters

Michael Lehnert1,2, Elena Kipf1, Franziska Schlenker1, Roland Zengerle1,2, Nadine Borst1,2, Felix von Stetten1,2

1: Hahn-Schickard, Freiburg, Germany; 2: Laboratory for MEMS Applications, Department of Microsystems Engineering - IMTEK, University of Freiburg, Germany

11:00am
-
12:30pm
MULTI2: Multi-Omics 2
Location: HS 14
Chair: Michael W Pfaffl, Technical University of Munich, Germany
 

Systems Medicine - or - What I learned about Arnold Schwarzenegger while studying breast cancer survival.

Jan Baumbach

Technical University of Munich, Germany



From Next Generation Sequencing to Next Generation Biomarkers: How Adaptive Focused Acoustics® is Transforming High-throughput Biology and Multi-omics Analyses

Nicolas Autret

Covaris, United Kingdom



Finding Signatures, Fingerprints and Prognostic Biomarkers in Large Biomedical Datasets: Computational and visual Approaches.

Andrej-Nikolai Spiess

UKE Hamburg, Germany

qPCR-DA1: qPCR Data Analysis 1
Location: HS 15
Chair: Stefan Rödiger, Brandenburg University of Technology Cottbus - Senftenberg, Germany
 

GeneGini: Assessment via the Gini Coefficient of Reference "Housekeeping" Genes and Diverse Human Transporter Expression Profiles.

Philip Day1, Stephen O'Hagan1, Marina Wright Muelas1, Emma Lundberg2, Douglas Kell1

1: University of Manchester, United Kingdom; 2: KTH Royal Institue of Technology, Stockholm, Sweden



GenEx – The Ultimate Software for Analysis of Transcriptomic Data.

Amin Forootan1, Björn Sjögreen1, Mikael Kubista2

1: Multid Analyses AB, Sweden; 2: TATAA Biocenter, Sweden



Why reporting Cq or delta-Cq is senseless.

Jan M Ruijter

Academic Medical Center, Amsterdam, the Netherlands, Netherlands, The

12:30pm
-
2:00pm
PO3: Wednesday Lunch Poster Session
Location: Lower Level
All posters will be displayed in parallel at all three poster sessions PO1, PO2 & PO3 => the poster viewing can be done from "Monday Evening Main Poster Session" till "Wednesday Lunch Poster Session"
LU3: Lunch Wednesday
Location: Mensa
2:00pm
-
4:00pm
AMDx2: Advanced Molecular Diagnostics 2
Location: HS 14
Chair: John Mackay, dnature Diagnostics & Research Ltd, New Zealand
 

“Saving The Bees Is Burning Down The House” - Triplex qPCR Using Dual-Target Assays For The Highly Pathogenic Bacteria American Foulbrood, Using Novel eDNA Methods.

John Mackay, Tammy Waters

dnature diagnostics & research Ltd, Gisborne, New Zealand



A Portable, Accurate, and Cost-Effective Strategy for Rapid On-Site Authentication and Characterization of Commercially Important Species and Pathogens Using Bio Molecular Systems’ MIC qPCR Cycler

Jesus Aquiles Sanchez, Lawrence Wangh

Thermagenix, Inc, Natick, Massachusetts, United States of America



Development Of An Event-Specific qPCR Method For Detection Of Genetically Modified Alfalfa

Patrick Guertler1, Lutz Grohmann2, Heike Naumann3, Melanie Pavlovic1, Ulrich Busch1

1: Bavarian Health and Food Safety Authority, Germany; 2: Federal Office of Consumer Protection and Food Safety, Germany; 3: Lower Saxony State Office for Consumer Protection and Food Safety, Germany



MyPOLS Biotec: Shaping DNA Polymerases For Your Needs

Moritz Welter1,2, Andreas Marx2, Ramon Kranaster1

1: myPOLS Biotec GmbH, Technologiezentrum Konstanz, Blarerstraße 56, 78462 Konstanz, Germany; 2: Chair of Organic Chemistry / Cellular Chemistry, University of Konstanz, Universitätsstraße 10, 78457 Konstanz, Germany

qPCR-DA2: qPCR Data Analysis 2
Location: HS 15
Chair: Jan M Ruijter, Academic Medical Center, Amsterdam, Netherlands, The
 

Integration of DNA Melting Curve Analysis In qPCR Data Analysis

Maurice J.B. van den Hoff1, Quinn D. Gunst1, Adrian Ruiz-Villalba2, Carl Wittwer3, Jan M. Ruijter1

1: Amsterdam UMC, location AMC, Depart. Medical Biology, Amsterdam, The Netherlands; 2: Foundation of Applied Medical Research, University of Navarra, Pamplona, Spain; 3: University of Utah Health Sciences Center, Department of Pathology, Salt Lake City, UT, USA



Fundamentals for the Automatic Classification of Quantitative PCR AmplificationCurves - A Biostatistical Approach

Stefan Rödiger1, Andrej-Nikolai Spiess2, Michał Burdukiewicz3

1: Brandenburg University of Technology Cottbus - Senftenberg, Germany; 2: University Medical Center Hamburg-Eppendorf, Germany; 3: Warsaw University of Technology, Poland



GEAR: The Genome Analysis Server Eases Wet-Lab Data Analysis

Tobias Rausch1, Markus Hsi-Yang Fritz2, Vladimir Benes1, Andreas Untergasser1,3

1: European Molecular Biology Laboratory, Genomics Core Facility, Heidelberg, Germany; 2: European Molecular Biology Laboratory, Genome Biology Unit, EMBL, Heidelberg, Germany; 3: Heidelberg University, Germany



DAILYqpcr – An Application For Revolutionizing Designing, Storing, And Analyzing QPCR Experiments

Stephan Pabinger, Anna Majewski, Manuela Hofner, Walter Pulverer, Priska Bauerstätter, Stefanie Eile, Julie Krainer, Andreas Weinhäusel, Klemens Vierlinger

AIT - Austrian Institute of Technology, Austria

4:00pm
-
4:30pm
FW: Farewell Session
Location: HS 14
Chair: Michael W Pfaffl, Technical University of Munich, Germany

Date: Thursday, 21/Mar/2019
9:00am
-
5:00pm
WS1: Workshop Day 1
Location: Workshop Rooms

Date: Friday, 22/Mar/2019
9:00am
-
5:00pm
WS2: Workshop Day 2
Location: Workshop Rooms