Logo qPCR dPCR & NGS 2017
 

Conference Agenda

Overview and details of the sessions of this conference. Please select a date or location to show only sessions at that day or location. Please select a single session for detailed view (with abstracts and downloads if available).

 
Session Overview
Date: Monday, 03/Apr/2017
10:00am
-
10:30am
Welcome
Location: Lecture hall 14
Chair: Michael W Pfaffl, TUM, Germany
Chair: Stephen Andrew Bustin, Anglia Ruskin University, United Kingdom
10:30am
-
12:30pm
LB1: Liquid Biopsies & Exosomes
Location: Lecture hall 14
Chair: Michael W Pfaffl, TUM, Germany
Chair: Stephen Andrew Bustin, Anglia Ruskin University, United Kingdom
 

Extracellular Vesicles and their Potential as Biomarkers and Therapeutics

Jan O Lötvall

University of Gothenburg, Sweden


Ultrasensitive mutation detection in liquid biopsies using SiMSen-Seq

Anders Ståhlberg

University of Gothenburg, Sweden


Droplet-based digital PCR for cancer patient follow up’

Valerie Taly

Université Paris Descartes/ INSERM/ CNRS, France


Exosomes - Advancing Liquid Biopsy Diagnostics

Mikkel Noerholm

Exosome Diagnostics, Germany

12:30pm
-
2:00pm
Lunch Monday
Location: University Mensa
2:00pm
-
6:00pm
LB2: Liquid Biopsies & Molecular Dx 1
Location: Lecture hall 14
Chair: Jo Vandesompele, Biogazelle, Belgium
Chair: An Hendrix, Ghent University, Belgium
 

mRNA capture sequencing enabled liquid biopsy precision oncology

Jo Vandesompele

Biogazelle, Belgium


Sensitive NGS method for the analysis of microbial and viral nucleic acid in cell free RNA

Martin Heine

NuGEN Technologies, United States of America


Identification Of Extracellular Vesicle-Specific Biomarkers

Jan Van Deun1,3, Glenn Vergauwen1,3, Pieter Mestdagh2,3, Jo Vandesompele2,3, Olivier De Wever1,2, An Hendrix1,2

1: Laboratory of Experimental Cancer Research, Department of Radiation Oncology and Experimental Cancer Research, Ghent University, Ghent, Belgium; 2: Department of Medical Genetics, Ghent University, Ghent, Belgium; 3: Cancer Research Institute Ghent, Ghent, Belgium


NGS and qPCR analysis of RNA and microRNA in Biofluids and Exosomes

Michael Thorsen

Exiqon, Denmark


Routine Next-Generation-Sequencing of Brain Tumors

Felix Sahm

University Hospital Heidelberg, Germany


BD Genomics: An Integrated Workflow For Single Cell Analysis That Helps To Uncover Tumor Heterogeneity

Wieland Keilholz

BD, Germany


Nucleosome association of cell-free DNA informs about gene expression

Peter Ulz1, Gerhard Thallinger2, Martina Auer1, Ricarda Graf1, Karl Kashofer3, Stephan Wenzel Jahn3, Luca Abete3, Gunda Pristauz4, Edgar Petru4, Jochen Geigl1, Ellen Heitzer1, Michael Speicher1

1: Institute of Human Genetics, Medical University Graz, Austria; 2: Institute of Molecular Biotechnology, University of Technology, Graz, Austria; 3: Institute of Pathology, Medical University of Graz, Graz, Austria; 4: Department of Obstetrics and Gynecology, Medical University of Graz, Graz, Austria

NGSDA: NGS Integrative Data Analysis
Location: Lecture hall 15
Chair: Vladimir Benes, EMBL, Germany
Chair: Daniel Simon Brewer, University of East Anglia, United Kingdom
 

Meta-OMIC reconstruction in the gut microbiome of wild primates: Impactions for human origins

Andres Gomez

J. Craig Venter Institute, United States of America


Gut Microbiota Sequencing: From Single Studies To Large-Scale Analysis

Thomas Clavel1, Ilias Lagkouvardos2

1: University Hospital RWTH Aachen, Germany; 2: ZIEL Institute, TU Munich, Freising, Germany


From qualitative to quantitative data in microbiome analysis: Using metagenomics for qPCR validation.

Michael Schloter1,2, Gisle Vestergaard1, Anne Schoeler1, Stefanie Schulz1, Fabian Bergkemper1

1: Helmholtz Zentrum München, Germany; 2: Technical University of Munich, Germany


Unravelling The Evolution Of Prostate Cancer Through Whole Genome Sequence Data.

Daniel S. Brewer1, David Wedge2,3, Christopher S.. Foster4, David Neal5, G. Steve Bova6, Andy G. Lynch5, Colin S. Cooper7,1, Ros Eeles7

1: Norwich Medical School, University of East Anglia, United Kingdom; 2: Cancer Genome Project, Wellcome Trust Sanger Institute, United Kingdom; 3: Oxford Big Data Institute & Oxford Centre for Cancer Gene Research, Wellcome Trust Centre for Human Genetics, United Kingdom; 4: HCA Pathology Laboratories, London, United Kingdom; 5: Cancer Research UK Cambridge Institute, Cambridge, United Kingdom; 6: University of Tampere and Tampere University Hospital, Finland; 7: Institute of Cancer Research, United Kingdom


Nanopore Sequencing - Entering New Next

Vladimir Benes, Jonathan Landry, Jonathon Blake, Bettina Haase, Dinko Pavlinic, Jan Provaznik

EMBL, Germany


Overcoming NGS analysis bottlenecks with a systematic, scalable system

Amit Sinha

Basepair, United States of America


Evaluation of Regulatory Small Variants in Human Genetics

Christian Zinser

Genomatix GmbH, Germany

6:00pm
-
10:00pm
GTR: Get-Together Reception @ Foyer
Location: Foyer
PO1: Main Postersession
Location: Foyer - lower level
All posters will be displayed in all three poster sessions (Monday evening and two lunch poster sessions). Poster setup is on Monday afternoon and all posters are available until Wednesday afternoon.
 

Ligation-free small RNA sequencing libraries from clinical samples using SMART® technology

Sara Gonzalez-Hilarion1, Nathalie Bolduc2, Simon Lee2, Rachel Fisch2, Kahraman Tanriverdi3, Evangelia Malahias3, Ekaterina Mikhalev3, Jane Freedman3, Andrew Farmer2

1: Takara Bio Europe, France; 2: Takara Bio USA; 3: University of Massachusetts Medical School, Department of Medicine, Division of Cardiovascular Medicine


Complete “Sample-to-Result” Highly Automated NGS and qPCR Workflow for Clinical Diagnostics

Elian Rakhmanaliev, Tatiana Ivanova, Yin Kum Ng, Alex Yeo, Harry Suhardi, Caroline Villy, Mei Qi Yee, Kevin Tan, Amanda Fan, Andy Soh, Pramila Ariyaratne, Si Kee Tan, Jocelyn Wong, Jiang Xia, Grace Tan, Jasmin Ang, John Ong, Charlie Lee, Gerd Michel, Wen Huang

Vela Diagnostics Pte. Ltd., Singapore


Gene Expression Variance As A Proxy For Chronological Age

Tong Shu Li, Michael Petrascheck, Andrew I. Su

The Scripps Research Institute, United States of America


Efficient Isolation on Vero.DogSLAMtag Cell Line and Whole Genome Characterization by Next Generation Sequencing of Dolphin Morbillivirus

Simone Peletto1, Claudio Caruso1, Cerutti Francesco1, Modesto Paola1, Cristina Biolatti1, Alessandra Pautasso1, Carla Grattarola1, Federica Giorda1, Sandro Mazzariol2, Walter Mignone1, Loretta Masoero1, Cristina Casalone1, Pier Luigi Acutis1

1: Istituto Zooprofilattico Sperimentale del Piemonte, Liguria e Valle d'Aosta, Turin, Italy; 2: University of Padova, Padua, Italy


Developing a potential biomarker set of miRNAs in packed RBCs to detect autologous blood doping

Anna Haberberger1, Veronika Mussack1, Benedikt Kirchner1, Irmgard Riedmaier1,2, Christian Wichmann3, Raymund Buhmann3, Georg Wittmann3, Reinhard Henschler3,4, Michael W. Pfaffl1

1: Technical University Munich, Animal Physiology and Immunology, Freising, Germany; 2: Eurofins Medigenomix Forensik GmbH, Ebersberg, Germany; 3: Ludwig-Maximilians University Hospital, Department of Transfusion Medicine, Cell Therapeutics and Hemostaseology, Munich, Germany; 4: Blutspende Zürich, Medizinischer Dienst, Zurich, Switzerland


Different Isolation Strategies of Exosomal Extraction Kits Have A Major Influence On Small RNA Capturing And Expression Profiles in Clinical Settings

Benedikt Kirchner1,2, Dominik Buschmann1,3, Stefan Kotschote4, Michael Bonin4, Marlene Reithmair3, Gustav Schelling5, Michael W. Pfaffl1

1: School of Life Sciences Weihenstephan, Technical University Munich, Germany; 2: Dr. von Hauner Childrens‘s Hospital, Ludwig Maximillian Universität, Munich, Germany; 3: Institute of Human Genetics, Ludwig-Maximilians-University Munich, Germany; 4: IMGM Laboratories GmbH, Planegg, Germany; 5: Department of Anesthesiology, Ludwig-Maximilians-University Munich, Germany


NEXTERA XT DNA Library Preparation on PIPETMAX

Laura B. Simdon1, Seth A. Hanson1, Brandon Converse2, Brendan Keogh2, Scott Monsma2, Tristan J. Berto1

1: Gilson Inc.; 2: Lucigen Corp.


SiMSen-Seq: Simple, Multiplexed, PCR-based Barcoding of DNA for Sensitive Mutation Detection using Sequencing

Stefan Filges1, Gustav Johansson1, Paul Krzyzanowski2, Tony E. Godfrey3, Anders Ståhlberg1

1: Department of Pathology, Sahlgrenska Cancer Center, Institute of Biomedicine, Sahlgrenska Academy at University of Gothenburg, Medicinaregatan 1F, 405 30 Gothenburg, Sweden; 2: Ontario Institute for Cancer Research, MaRS Centre, 661 University Avenue, Suite 510, Toronto, Ontario, Canada M5G 0A3; 3: Department of Surgery, Boston University School of Medicine, 700 Albany Street, Boston, MA 02118, USA


TempO-Seq: High-Throughput Surrogate and Whole Transcriptome Targeted Gene Expression Analysis

Harper VanSteenhouse, Pete Shepard, Milos Babic, Joanne Yeakley, Bruce Seligmann

BioSpyder Technologies, United States of America


SIRV SUITE: RNA-Seq Quality Metrics and Comparison Platform

Igor Holländer, Patrick Schagerl, Lukas Paul, Torsten Reda

Lexogen GmbH, Austria


Suppression Before Amplification Of Highly Abundant mRNA To Profile Gene Expression From Microdissected Mammary Epithelial Cells

Claudia Bevilacqua1, Nicolas Crapart1,2, Cathy Hue-Beauvais1, Barbara Brandao1, Sophie Lemoine3, Fanny Coulplier3, Lucile Broseus4, Patrice Martin1

1: GABI, INRA, AgroParisTech, Université Paris-Saclay, Jouy-en-Josas; 2: EXCILONE, Elancourt, 78990 France; 3: Plateforme de Génomique, Institut de Biologie de l’ENS, IBENS, Paris F-75005, France; 4: INRA, UMR1213 Herbivores, F-63122 Saint-Genès Champanelle, France


Implementation of a new automated Sample Quality Control tool in a Whole Exome Sequencing workflow

Elisa Viering1, Jana Petersen2, Marie Beckhaus2, Jana Molitor1, Stephan Wolf2

1: Agilent Technologies, Inc. Waldbronn, Germany; 2: DKFZ Genomics and Proteomics Core Facility, High Throughput Sequencing Unit, Heidelberg, Germany


A SMARTer Approach to Profiling the Human T-Cell Receptor Repertoire Using the ICELL8 Single-Cell System

Sara Gonzalez-Hilarion1, Sarah Taylor2, Thomas Schaal3, Jude Dunne3, Maithreyan Srinivasan3, Andrew Farmer2

1: Takara Bio Europe, France; 2: Takara Bio USA; 3: WaferGen Bio-Systems, Inc.


When the promises and perils of advanced molecular diagnostics unexpectedly go hand in hand: Genomic Analysis of Uterine Lavage Detects Early Endometrial Cancers and Reveals a Prevalent Cancer-Driver Mutation Landscape in Women without Cancer

John A. Martignetti1,2, Navya Nair1, Olga Camacho-Vanegas1, Dmitry Rykunov1, Matthew Dashkoff1, Sandra Camacho1, Timothy Harkins3, Cassie Schumacher3, Jon Irish3, Elena Pereira1, Sviatoslav Kendall1, Tamara Kalir1, Nimesh Nagarsheth1, Robert Sebra1, Boris Reva1, Peter Dottino1

1: Icahn School of Medicine at Mount Sinai, NY, United States of America; 2: Laboratory of Translational Research, Western Connecticut Health Network, Danbury, CT; 3: Swift Biosciences, Ann Arbor, MI


Influence of Genome Update and Methodology on Biomarker Discovery – RNA-Seq study in chicken

Silvia Fibi1, Candida Vaz2, Bertrand Grenier1, Gerd Schatzmayr1, Vivek Tanavde2

1: Biomin Research Center, Biomin Holding GmbH, Technopark 1A, A-3430 Tulln; 2: Bioinformatics Institute, 30 Biopolis Street, Singapore 138671


Avoiding DNA adsorption to tube walls in NGS sample preparation

Emily Flowers, Hanna Oldfield, Gerrit Gutzke

4titude Ltd, Germany


An Information System to Support Genetically Modified Organisms Analysis Strategies in Turkey

Remziye Yılmaz, Ceren C. Satır

Hacettepe University, Turkey


Identification and quantification of genetically modified organisms (GMO) from high throughput sequencing data

Bjørn Spilsberg, Karin Lagesen, Anja Bråthen Kristoffersen, Arne Holst-Jensen

Norwegian Veterinary Institute, Norway


Optimized Analysis Procedure For Monitoring Transgenic Rapeseed Along Transportation Routes In Bavaria

Larissa Murr, Patrick Guertler, Melanie Pavlovic, Ottmar Goerlich, Ulrich Busch

Bavarian Health and Food Safety Authority, Oberschleißheim, Germany


Development Of Specific qPCR Assays For The Detection Of Genetically Modified Alfalfa J101, J163 and KK179

Patrick Guertler1, Melanie Pavlovic1, Heike Naumann2, Ulrich Busch1

1: Bavarian Health and Food Safety Authority, Oberschleissheim, Germany; 2: Lower Saxony State Office for Consumer Protection and Food Safety, Braunschweig, Germany


GMO Analysis of Soy Lecithin and Products Containing Soy Lecithin

Danica Milinkov Guljaš, Jelena Hrubik, Gordana Nović

SP Laboratorija, Serbia


Biocev GeneCore: More Than Just Gene Expression Profiling

Lucie Langerova, David Švec

Institute of Biotechnology, Czech Republic


Automated, high-throughput quantitative PCR for accurate and economical copy number determination

Stephan Bauer

LGC


Comparison of Manual vs. Automated qPCR Reaction Plate Setup

Tristan J Berto, Amber Mael, Laura Simdon, Michael Herren

Gilson Inc.


Opening Pandora’s box – unmasking the need for a continuous sample processing control for qPCR based fecal marker analysis

Rita Linke1, Sibel Zeki1,4, René Mayer1,2, Katharina Keiblinger5, Simone Ixenmaier1,2, Robert Mach6, Regina Sommer2,7, Georg Reischer1,8, Andreas Farnleitner1,2,3

1: TU Wien, Institute of Chemical and Biological Engineering, Research Group Environmental Microbiology and Molecular Diagnostics 166/5/4, 1060 Vienna, Austria; 2: Interuniversity Cooperation Centre for Water and Health: www.waterandhealth.at; 3: Karl Landsteiner University of Health Sciences, Research Unit Water Quality and Health, 3500 Krems, Austria; 4: Department of Marine Environment, Institute of Marine Sciences and Management, Istanbul University, Istanbul, Turkey; 5: Institute of Soil Research, Department of Forest and Soil Sciences, University of Natural Resources and Life Sciences Vienna, Austria; 6: TU Wien, Institute of Chemical and Biological Engineering, 1060 Vienna, Austria; 7: Medical University Vienna, Clinical Institute of Hygiene and Medical Microbiology, Water Hygiene, Vienna, Kinderspitalgasse 15, Austria; 8: IFA Tulln, Center for Analytical Chemistry, Department of Agrobiotechnology, Working Group Molecular Diagnostics, 3430 Tulln, Austria


Influence of PCR consumables on the accuracy of real-time PCR experiments

Gerrit Gutzke, Hanna Oldfield, Emily Flowers

4titude Ltd, Germany


Cell-free DNA assessment for clinical purposes: optimization of blood collection and DNA extraction methodologies

Jos Drabbels, Berit Kemps, Marijke Spruyt, Frans Claas, Michael Eikmans

Leiden University Medical Center, The Netherlands


Compatibility of Magnetic Nanoparticles with Polymerase Chain Reaction

Ayse Beyza Aysan1, Zdeněk Knejzlík1,2, František Štěpánek1

1: University of Chemistry and Technology, Prague, Czech Republic; 2: Institute of Organic Chemistry and Biochemistry of the Czech Academy of Sciences of the Czech Republic


Conception And Optimization Of A Detection Method Of Filovirus Species By Combining The Use Of Inosines And HRM Analysis.

Graziella Penot, Laure Barbier, Charles Chapus, Thomas Poyot

IRBA, France


Evaluation of SNPs based on DNA extracted from urine as part of the development and use of a kit of non-invasive biomarkers to monitor respiratory health of young children

Sarah Nauwelaerts1,2, Koen De Cremer2, Alfred Bernard3, Nancy Roosens1, Sigrid De Keersmaecker1

1: Platform Biotechnology and Molecular Biology DO Expertise, service provision and customer relations, Scientific Institute of Public Health, Brussels, Belgium; 2: Health and Environment DO Food, medicines and consumer safety, Scientific Institute of Public Health, Brussels, Belgium; 3: Louvain Centre for Toxicology and Applied Pharmacology, Université Catholique Louvain Woluwe, Brussels, Belgium


Modaplex, a novel technology for the multiplex analyses of CpG and miRNA signatures in a single qPCR run

Diana Alejandra Grohme1, Kerstin Korn1, Juliane Rothe2, Ruth Merkle3, Rongxi Yang3

1: Biotype Innovation GmbH, Moritzburger Weg 67, 01109 Dresden, Germany; 2: Biotype Diagnostic GmbH, Moritzburger Weg 67, 01109 Dresden, Germany; 3: Department of Gynecology and Obstetrics, University Women's Hospital of Heidelberg, 69120 Heidelberg, Germany


Molecular Diagnosis of Toxoplasmosis in Serologically Positive Pregnant Women

Ouassila Aouacheri1, Hajira Berredjem1, Meryem Benlaifa1, Rafika Bardi2

1: Applied Biochemistry and Microbiology Laboratory, Department of Biochemistry, Faculty of Sciences, Badji Mokhtar University- Annaba,Algeria; 2: Laboratory of Immunology, EPS Charles Nicolle, Tunis.


QRT-PCR Methods to evaluate the seasonal variations of relative expression of testicular LhcgR mRNA in adult male Libyan Jird (Meriones libycus)

Radia Boufermes

BADJI Mokhtar University of Annaba, Algeria


Real Time PCR to Evaluate the Effect of the N-Acylsulfonamide Bis-oxazolidin-2-ones on Toxoplasma gondii’s Infected Swiss Mice

Hajira Berredjem1, Meryem Benlaifa2, Radia Buasla3, Malika Berredjem3, Rafika Bardi4

1: University of Badji Mokhtar, Department of Biochemistry, Laboratory of Applied Bio-chemistry and Microbiology, Annaba, Algeria; 2: University of Badji Mokhtar, Department of Biology, Laboratory of Cell Toxicology, Annaba, Algeria; 3: University of Badji Mokhtar, Laboratory of Applied Organic Chemistry, Synthesis of Biomolecules and Molecular Modelling Group, Annaba, Algeria; 4: Laboratory of Immunology, EPS Charles Nicolle, Tunis


A Quantitative Real-Time PCR For Pharmacokinetic Analysis Of The Immunotherapeutic TLR9 Agonist Lefitolimod

Detlef Oswald1, Kerstin Kapp1, Burghardt Wittig2, Manuel Schmidt1

1: MOLOGEN AG, Germany; 2: Foundation Institute Molecular Biology and Bioinformatics, Freie Universität Berlin


Expression and Localization of the Thrombospondin Family Members During Corpus Luteum Formation and Function in the Bovine Ovary

Bajram Berisha1,2, Dieter Schams2, Daniela Rodler3, Fred Sinowatz3, Michael W. Pfaffl2

1: Faculty of Agriculture and Veterinary, University of Prishtina, Prishtinë, Kosovo.; 2: Institute of Animal Physiology and Immunology Weihenstephan, Technical University of Munich, Freising, Germany.; 3: Institute of Anatomy, Histology and Embryology, Ludwig Maximilian University of Munich, Munich, Germany.


Gene Expression Signatures Of Bovine In Vitro Embryos Produced At The Air-liquid Interphase On Oviduct Epithelial Cells

Vera A. van der Weijden1, Shuai Chen2, Susanne E. Ulbrich1, Jennifer Schoen2

1: ETH Zurich, Animal Physiology, Institute of Agricultural Sciences, Switzerland; 2: Leibniz Institute for Farm Animal Biology, FBN Dummerstorf, Germany


Deregulated Genes in Hematopoietic Stem Cells Isolated from Spleen and Peripheral Blood of Patients with Myelofibrosis

Paolo Catarsi1, Francesca Cordero2,4, Giulio Ferrero2,3,4, Marco Beccuti4, Valentina Poletto1, Elisa Bonetti1, Laura Villani1, Margherita Massa5, Gabriela Fois1, Rita Campanelli1, Umberto Magrini1, Vittorio Rosti1, Giovanni Barosi1

1: Center for the Study and Treatment of Myelofibrosis, Biotechnology Research Area, Fondazione IRCCS Policlinico "San Matteo",Pavia, Italy; 2: Center for Molecular Systems Biology, University of Turin, Turin, Italy; 3: Department of Clinical and Biological Sciences, University of Turin, Turin, Italy; 4: Department of Computer Science, University of Turin, Turin, Italy; 5: Biotechnology Research Area, Fondazione IRCCS Policlinico "San Matteo",Pavia, Italy


A Microbead-based Assay for the Real-Time Detection of Multiple HPV Genotypes

Roy-Arne Senkel1, Claudia Deutschmann1, Werner Lehmann2, Peter Schierack1, Stefan Rödiger1

1: Brandenburg University of Technology Cottbus–Senftenberg, Senftenberg, Germany; 2: Attomol GmbH, Lipten, Germany


VitiPatho a qPCR-Method for the prediction of thresholds for fungizide application.

Bernhard Setzer1, Rene Fuchs2

1: BS-diagnostik, Germany; 2: State Institute for Viticulture and Oenology of Freiburg, Germany


A Novel High-multiplex Homogeneous PCR Assay Format

Søren Morgenthaler Echwald

Anapa Biotech A/S, Denmark


Detection Of EGFR Mutations In Cell-Free DNA From Patients Affected By Lung Cancer: Prenalytical And Analytical Aspects

Francesca Salvianti, Francesca Malentacchi, Francesca Damiani, Irene Mancini, Mario Pazzagli, Pamela Pinzani

Scienze Biomediche, Sperimentali e Cliniche, Italy


Allelic Frequency of a 24-bp Duplication in Exon 10 of the CHIT1 Gene in the General Iranian Population by HRM Analysis

Behrooz Motlagh1, Mohammad Taghikhani2, Nasim Abedimanesh3

1: Zanjan University of Medical Sciences, Iran, Islamic Republic of; 2: Tarbiat Modares University, Iran, Islamic Republic of; 3: Tabriz University of Medical Sciences, Iran, Islamic Republic of


Development of an R Package for qPCR Data Management with the Real-time PCR Data Markup Language

Konstantin Blagodatskikh1, Michał Burdukiewicz2, Andrej-Nikolai Spiess3, Stefan Rödiger4

1: Evrogen JSC, Moscow, Russia; 2: Department of Genomics, Faculty of Biotechnology, University of Wrocław, Wrocław; 3: University Medical Center Hamburg-Eppendorf, Hamburg, Germany; 4: Institute of Biotechnology, Brandenburg University of Technology Cottbus–Senftenberg, Senftenberg, Germany


A comparison of nonlinear fitting methods to raw qPCR amplification curves: performance, caveats and implications for Cq and E estimation.

Andrej-Nikolai Spiess1, Stefan Rödiger2, Joel Tellinghuisen3

1: Department of Andrology, University Hospital Hamburg-Eppendorf, Germany; 2: Brandenburg University of Technology Cottbus–Senftenberg, Senftenberg, Germany; 3: Department of Chemistry, Vanderbilt University, Nashville, Tennessee 37235, USA


The Role of Amplification Efficiency and its Uncertainty in Assessing Relative Expression by the ΔΔCq Method: A Nonlinear Least-Squares Approach that Overcomes Deficiencies in a Widely Used SAS Program

Joel Tellinghuisen

Vanderbilt University, United States of America


Urine microRNAs as potential diagnostic and prognostic biomarkers of urinary bladder cancer

Jaroslav Juracek1,2, Sona Klusova1, Michal Stanik2, Jan Dolezel2, Ondrej Slaby1,2

1: Central European Institute of Technology, Czech Republic; 2: Masaryk Memorial Cancer Institute, Czech Republic


Is Quantitative microRNA Expression Profiling Using Fluidigm Microfluidic Dynamic Arrays Reliable? Taqman microRNA Assay Evaluation.

Vlasta Korenková1, Jan Král2, Veronika Vymetálková2, Vendula Novosadová1, Lucie Langerová1, Pavel Vodička2, Jana Slyšková2

1: Institute of Biotechnology, Czech Republic; 2: Institure of Experimental Medicine, Czech Republic


Analysis of microRNA isoforms (isomiRs) in Galaxy and their differences in expression levels

Jochen Bick, Susanne E. Ulbrich, Stefan Bauersachs

ETH Zurich, Switzerland


Development of miRNA Profiling Capacity Using Reverse Transcription–Quantitative PCR (RT-qPCR) by Prestizia, A miRNA Platform For Biomarkers Signature Discovery

Marjorie Monleau, Gilles Vieira, Laurent Tosolini, Odile Prigneau

Prestizia, France


Two-tailed RT-qPCR: a Novel Method for Highly Accurate miRNA Quantification

Peter Androvic1, Lukas Valihrach1, Julie Elling2, Robert Sjoback2, Mikael Kubista1,2

1: Institute of Biotechnology CAS, Czech Republic; 2: TATAA Biocenter AB, Sweden


Tetraspanin CD9 Modifies the RNA Cargo of Prostate Extracellular Vesicles

Helen Jankowski1, Belinda Goldie1,2, Joshua Brzozowski1, Benjamin Munro1, Christopher Scarlett1, Kathryn Skelding1, Judith Weidenhofer1

1: University of Newcastle and Hunter Medical Reasearch Institute (HMRI), Australia; 2: nstitute for Integrated Cell-Material Sciences (iCeMS), Kyoto University, Kyoto, Japan


EXÖBead: an efficient method of isolating exosomes from small sample volumes without ultracentrifugation

Dapi Chiang1, Dominik Buschmann2, Benedikt Kirchner2, Michael W. Pfaffl2

1: Biovesicle Inc., Taiwan; 2: Division of Animal Physiology and Immunology, School of Life Sciences Weihenstephan, Technical University Munich, Germany


Droplet Digital PCR: analysis approach for the detection and absolute quantification of lactic acid bacteria

Antje-Kristin Mommsen, Stephan Scholtz

DuPont Danisco Deutschland GmbH, Germany


Clinical samples collected in Streck Cell-Free DNA blood collection tubes are suitable for liquid biopsy testing using BEAMing digital PCR

Annette Nocon1, Inga Medina Diaz1, Makbule Kobilay2, Dirk Skowasch2, Stefanie Held2, Claudia Stamm1, Frank Diehl1, Stefan Holdenrieder2, Frank Holtrup1

1: Sysmex Inostics GmbH, Germany; 2: University Hospital Bonn, Germany


Digital PCR quantification of the 7S DNA per mitochondrial genome proportion in non-invasive sources of human cell types

Philipp Steindorf1, Martin Hofer1, Roman Ziegler1, Bernard Wallner2, Ralf Steinborn1

1: Genomics Core Facility, VetCore, University of Veterinary Medicine, Veterinärplatz 1, Vienna, Austria; 2: Department of Behavioural Biology, University of Vienna, Vienna, Austria


Poisson Plus Quantification for the QuantStudio™ 3D Digital PCR System

Nivedita Majumdar, Swapnonil Banerjee, Thomas Wessel, Mike Pallas, Patricia Hegerich

Thermofisher Scientific, United States of America


Detection of TMPRSS2:ERG Fusion Transcript Using TaqMan Assays and the QuantStudio 3D Digital PCR System

Alice Rödel1,2, Marion Laig3, Arndt Schmitz1

1: Bayer AG, Berlin, Germany; 2: Beuth University of Applied Sciences, Berlin Germany; 3: Thermo Fisher Scientific, South San Francisco, CA, United States


Toward elucidation of the biological function of S100 calcium binding protein A9 in monocytes using single cell sorting and qPCR methodologies

Jianxin Yang, Carin van der Keur, Frans Claas, Michael Eikmans

LUMC,The Netherlands


Determining the Limit of Detection of Rare Targets Using Digital PCR

Nivedita Majumdar, Thomas Wessel, Marion Laig, Brian Ho, David Keys

Thermofisher Scientific, United States of America


Single Cell Gene Expression Profiling in Breast Cancer Cells with the Her2/neu Gene Knockout by CRISPR-Cas9

Xiaoyang {Alice} Wang1, Chip Lomas1, Michael A. Tycon1, Craig Betts2, Wieland Keilholz2, Suzanne Weaver2

1: BD Biosciences; 2: BD Genomics


T cell differentiation dynamics profiled by massively parallel single cell RNA capture and highly multiplexed PCR

Christina Chang, Eleen Shum, Nidhanjali Bansal, Joanna Dreux, Gretchen Lam, Jue Fan, Wieland Keilholz, Christina Fan

BD Genomics


High Throughput Single-Cell Gene Expression Analysis Reveal Cell Type Specific Differences in Response to Different Human Peripheral Blood Preservation Protocols

Kimberly R. Cordes Metzler, Nidhanjali Bansal, Gretchen Lam, Jue Fan, Wieland Keilholz, Christina Fan

BD Genomics


Preamplification using dUTP and Cod UNG allows for reliable detection and quantification of limited samples sizes in the presence of contaminating DNA amplicons

Daniel Andersson1, David Svec1,2, Cathrine Pedersen3, Jørn Remi Henriksen3, Anders Ståhlberg1

1: Sahlgrenska Cancer Center, Department of Pathology, Sahlgrenska Academy at University of Gothenburg, Box 425, 40530 Gothenburg, Sweden; 2: Institute of Biotechnology, Academy of Sciences of the Czech Republic, Videnska 1083, Prague 4, 14221, Czech Republic; 3: ArcticZymes AS, Sykehusveien 23, 9019 Tromsø, Norway


Achieving Unparalleled Sensitivity and Reproducibility in Single-Cell Transcriptomics

Sara Gonzalez-Hilarion1, Kazuo Tori2, Cynthia Chang2, Yevgeniy Gindin2, Magnolia Bostick2, Andrew Farmer2

1: Takara Bio Europe, France; 2: Takara Bio USA


Resolving Molecular Networks and Dynamics Involved in CD8+ T-cells Function and Differentiation in Acute and Chronic Infections on a Single-Cell Level

Kristiyan Kanev, Patrick Roelli, Dietmar Zehn

Division of Animal Physiology and Immunology, Technical University of Munich, Germany


Combining Microgenomics Methods To Study How Gut Microbiota Influences Gene Expression In The Hypothalamo-Pituitary-Adrenal Axis In Rats.

Patricia Anglade1, Elise Maximin1, Nicolas Crapart2,3, Jad Abou-Ghantous4, Marie-Noelle Rossignol2, Philippe Gerard1, Patrice Martin2, Claudia Bevilacqua2, Sylvie Rabot1, Bénédicte Goustard1

1: Micalis Institute, INRA, AgroParisTech, Université Paris-Saclay, Jouy-en-Josas, France; 2: GABI-Plareforme @BRIDGe, INRA, AgroParisTech, Université Paris-Saclay, Jouy-en-Josas; 3: EXCILONE, Elancourt, 78990 France; 4: MGP MetaGenoPolis, INRA, Université Paris-Saclay, Jouy-en-Josas, France


TaqMan™ Assays for Detection of Fusion Transcripts

Fangqi Hu, Pius Brzoska, Xiaoqing You

Thermo Fisher Scientific, United States of America


QuantSeq 3’ mRNA Sequencing Bridges the Gap Between Microarrays and Conventional RNA-Seq Methods and Yields Good Correlation Between High and Low Quality (FFPE) Samples

Petra Kubala, Musashi Tsujita, Michael Ante, Irmlind Gabler, Jekaterina Aleksejeva, Birgit Steinmetz, Dalia Daujotyte, Lukas Paul, Pamela Moll

Lexogen GmbH, Austria


QuantSeq as an Efficient Tool for Gene Expression Profiling and Targeted Sequencing: High Multiplexing,Sensitivity, and Reproducibility with Lowest Costs and Efforts

Pamela Moll, Musashi Tsujita, Michael Ante, Birgit Steinmetz, Lukas Paul, Irmlind Gabler, Jekaterina Aleksejeva, Dalia Daujotyte, Alexander Seitz, Torsten Reda

Lexogen GmbH, Austria


Application of allele-specific qPCR and NGS for detecting epigenetic adaptations at the FKBP5 locus: Relevance for stress-related diseases

Tobias Wiechmann1, Torsten Klengel2, Simone Röh1, Darina Czamara1, Susann Sauer1, Theo Rein1, Nadine Provençal1, Elisabeth B. Binder1,3

1: Dep. of Translational Research in Psychiatry, MPI of Psychiatry, Munich, Germany; 2: Dep. of Psychiatry, McLean Hospital, Harvard Medical School, Belmont, MA, USA; 3: Dep. of Psychiatry and Behavioral Sciences, Emory University Medical School, Atlanta, GA, USA


Overexpression of MiR-590, MiR-19a, and MiR-19b in Human Breast Carcinoma Is Associated with Poor Differentiation of Tumors, Negative Expression of Hormonal Receptors, and Worse Survival in Patients Treated by Chemotherapy

Veronika Brynychova1,2,3, Radka Vaclavikova1, Viktor Hlavac1,2,3, Marcela Mrhalova4, Roman Kodet4, Pavel Soucek1,3

1: The National Institute of Public Health, Czech Republic; 2: Third Faculty of Medicine, Charles University, Prague, Czech Republic; 3: Biomedical Centre, Medical Faculty, Charles University, Pilsen, Czech Republic; 4: Department of Pathology & Molecular Medicine, Second Faculty of Medicine, Charles University & Motol University Hospital, Prague, Czech Republic


A new core facility for high throughput qPCR : qPCR-HD-GPC

Juliette Pouch1,5, Elise Diaz1,2, Amine Ali Chaouche1, Quentin Viautour1,3, Auguste Genovesio3, Bertrand Ducos1,2,4

1: High Throughput qPCR Facility, Institut de Biologie de l'ENS (IBENS), Paris, France; 2: LPS-ENS, CNRS, PSL Research University, Paris, France; 3: Bioinformatic Facility, Institut de Biologie de l'ENS (IBENS), Paris, France; 4: Laser Microdissection Facilty, CIRB Collège de France, Paris, France; 5: Genomic Platform, IBENS, Paris, France


Detection Of Solid Tumor-Specific Mutant Transcripts In Blood Derived Extracellular Vesicles.

Silvia Rita Vitale1,3, Jean Helmijr1, Lisanne van Dessel1, Nick Beije1, Michelle van der Vlugt-Daane1, Martijn PJK Lolkema1, Maurice Jansen1, Stefan Sleijfer1, Anieta M Sieuwerts1,2, John WM Martens1,2

1: Erasmus MC Cancer Institute, Erasmus University Medical Center, Dept. of Medical Oncology, The Netherlands; 2: Cancer Genomics Netherlands, Rotterdam, The Netherlands; 3: University of Catania, Dept. of Clinical and Molecular Medicine, Italy


Profiling of Circulating MicroRNA in Patients Presenting with ST-segment Elevation Myocardial Infarction

Agata Maciejak1, Marek Kiliszek2, Agnieszka Segiet3, Grzegorz Opolski4, Monika Gora1, Beata Burzynska1

1: Institute of Biochemistry and Biophysics Polish Academy of Sciences, Warsaw, Poland; 2: Department of Cardiology and Internal Diseases, Military Institute of Medicine, Warsaw, Poland; 3: 1st Faculty of Medicine, Medical University of Warsaw, Warsaw, Poland; 4: 1st Chair and Department of Cardiology, Medical University of Warsaw, Warsaw, Poland


HiFit : robust data analysis method for High throughput qPCR

Quentin Viautour1,6, Juliette Pouch2,3,6, Amine Ali Chaouche2,3,6, Elise Diaz2,4,6, Mathieu Bahin1,6, Bertrand Ducos2,4,5, Auguste Genovesio1,6

1: Scientific Center for Computational Biology, Institut de Biologie de l'ENS, Paris, France; 2: High Throughput qPCR Facility, Institut de Biologie de l'ENS, Paris, France; 3: Genomic Platform, Institut de Biologie de l'ENS, Paris, France; 4: LPS-ENS, CNRS UMR8550, Paris, France; 5: Laser Microdissection Facility, CIRB Collège de France, Paris, France; 6: PSL Research University


The Development of Genomic Reference Materials for Quantitative Diagnostics

A. Pia Sanzone1, Jennifer Boyle1, Malcolm Hawkins2, Paul Metcalfe2, J. Ross Hawkins1

1: Division of Advanced Therapies, National Institute of Biological Standards and Control (NIBSC); 2: Division of Biotherapeutics, National Institute of Biological Standards and Control (NIBSC)


Impact of GSTM1 Polymorphism on the Susceptibility to Prostate Cancer in the Algerian Population

Maroua Benabdelkrim1, Hajira Berredjem1, Asma Bourefis1, Omar Djeffal2, Vincenzo Ciminale3

1: University of Badji Mokhtar, Department of Biochemistry, Laboratory of Applied Biochemistry and Microbiology, Annaba, Algeria.; 2: Cabinet médical privé d’uro-chirurgie, Annaba, Algérie.; 3: Laboratory of clinical and experimental oncology, university of Padova, Italy

Date: Tuesday, 04/Apr/2017
8:30am
-
12:30pm
dPCR 1: digital PCR 1
Location: Lecture hall 14
Chair: Jim Huggett, LGC & University of Surrey, United Kingdom
Chair: Afif M. Abdel Nour, Holy Spirit University of Kaslik, Lebanon (Lebanese Republic)
 

Unprecedented accuracy when using digital PCR

Jim Huggett

LGC & University of Surrey, United Kingdom


Liquid profiling of RNA and DNA for treatment response prediction and monitoring of cancer patients

Christof Winter

TU München, Germany


RT-Digital PCR for the evaluation of residual disease in chronic myeloid leukaemia

Mary Alikian1,2, Olga Tatarinova1, Alistair Reid1, Jane Apperley2,3, Letizia Foroni2,3

1: Imperial Molecular Pathology, Imperial Healthcare Trust, Hammersmith Hospital, London, UK; 2: Centre for Haematology, Faculty of Medicine, Imperial College London, London, UK; 3: Clinical Haematology, Imperial College Healthcare NHS Trust, London, UK


Calibrating Quantitative Measurements of HIV DNA Using Digital PCR

Eloise Busby1, Alexandra Whale1, R. Bridget Ferns2, Paul R Grant3, Gary Morley1, Jonathan Campbell1, Carole Foy1, Eleni Nastouli3,4, Jim Huggett1,5, Jeremy A Garson2,6

1: Molecular and Cell Biology Team, LGC, Teddington, UK; 2: Department of Infection, Division of Infection and Immunity, University College London, London, UK; 3: Department of Clinical Virology, University College London Hospital NHS Foundation Trust, and the UCL/UCLH NIHR Biomedical Research Centre, London, UK; 4: Department of Population Policy and Practice, UCL GOS Institute of Child Health, London, UK; 5: School of Biosciences & Medicine, Faculty of Health & Medical Science, University of Surrey, Guildford, GU2 7XH, UK; 6: National Transfusion Microbiology Laboratories, NHS Blood and Transplant, Colindale, London, UK


Monitoring EGFR mutations in lung cancer patients using 3-color Crystal Digital PCR.

Magali Droniou

Director of Applications, Stilla Technologies, France


Transferring qPCR Methods into a Droplet Digital PCR Format – Experiences from Official Food and Feed Control in GMO Analysis

Sven Pecoraro

Bavarian Health and Food Safety Authority, Germany


Simultaneous quantification of DNA copy number and transcripts by Selfie-dPCR.

Petar Podlesniy1, Ramon Trullas2

1: Institute of Biomedical Research of Barcelona, Barcelona, Spain; 2: Institute of Biomedical Research of Barcelona, Barcelona, Spain

LB3: Liquid Biopsies & Molecular Dx 2
Location: Lecture hall 15
Chair: Kenneth W Witwer, Johns Hopkins University School of Medicine, United States of America
Chair: Michael W Pfaffl, TUM, Germany
 

Extracellular Vesicle Subsets and Sampling Strategies for Nucleic Acid Profiling

Kenneth W Witwer

Johns Hopkins University School of Medicine, United States of America


Cellular and Extracellular miRNAs are Blood Compartment-Specific Diagnostic Targets for Detection and Survival Prediction in Sepsis

Marlene Reithmair1, Dominik Buschmann1,2, Melanie Märte3, Benedikt Kirchner2, Daniel Hagl3,4, Ines Kaufmann4, Martina Pfob1, Alexander Chouker3, Ortrud Steinlein1, Michael W. Pfaffl2, Gustav Schelling3

1: Institute of Human Genetics, University Hospital, Ludwig-Maximilians-University Munich, Germany; 2: Division of Animal Physiology and Immunology, TUM School of Life Sciences Weihenstephan, Technical University Munich; 3: Department of Anesthesiology, University Hospital, Ludwig-Maximilians-University, Munich; 4: Department of Anaesthesiology, Neuperlach Hospital, City Hospitals of Munich, Munich


Diagnostic Performance of Plasma DNA Methylation Profiles in Lung Cancer, Pulmonary Fibrosis and COPD

Andreas Weinhäusel1, Matthias Wielscher1, Klemens Vierlinger1, Rolf Ziesche2, Andrea Gsur2, Christa Noehammer1

1: AIT Austrian Institute of Technology GmbH, Molekulare Diagnostik, Austria; 2: Medical University Vienna, Austria


HP’s Advanced Microfluidic Technology

Alexander N. Govyadinov

HP Incorporated, United States of America


NGS analysis of the Liquid biopsy in metastatic breast cancer patients: a pilot study.

Pamela Pinzani, Francesca Salvianti, Mario Pazzagli

University of Florence, Italy


Validation Of Clinical Diagnostic Real-Time PCR Kits On AriaMX-DX

Anna Gani, Silvia Mason

AB ANALITICA, Italy


Going to the limits of Multiplex qPCR.

Olfert Landt

TIB MOLBIOL, Berlin, Germany

12:30pm
-
2:00pm
PO2: Postersession 2
Location: Foyer - lower level
All posters will be displayed in all three poster sessions (Monday evening and two lunch poster sessions). Poster setup is on Monday afternoon and all posters are available until Wednesday afternoon.
Lunch Tuesday
Location: University Mensa
1:00pm
-
2:00pm
LTS-IDT: Technical Lunch-time Seminar by IDT
Location: Lecture hall 14
Chair: Caifu Chen, Integrated DNA Technologies, United States of America
 

Novel multiplex rhPCR and its applications in amplicon sequencing.

Caifu Chen

Integrated DNA Technologies, United States of America


rhAmpTM A Better and Cost-Effective New SNP Genotyping Solution.

Kristin Beltz

Integrated DNA Technologies, Inc., United States of America

LTS-Genex: Technical Lunch-time Seminar by MultiD & TATAA
Location: Lecture hall 15
Chair: Mikael Kubista, TATAA Biocenter AB, Sweden
 

GenEx ­ -- the ultimate tool for qPCR data analysis.

Mikael Kubista1, Amin Forootan2

1: TATAA Biocenter AB, Göteborg, Sweden; 2: MultiD Analyses AB, Göteborg, Sweden

2:00pm
-
6:00pm
NGS: Next Generation Sequencing
Location: Lecture hall 14
Chair: Justin O'Grady, University of East Anglia, United Kingdom
Chair: Dominik Buschmann, Technical University Munich, Germany
 

Improving the Diagnosis and Management of Serious Infection Using Nanopore Metagenomic Sequencing

Justin O'Grady

University of East Anglia, United Kingdom


Applications of an in vitro Experimental Model for a Systems Level Understanding of Health and Disease States of the Human Oral Microbiome

Anna Elisabet Edlund1, Shibu Yooseph2, Wenyuan Shi3, Xuesong He3, Jeffrey Scott McLean4

1: J Craig Venter Institute, Genome Medicine Group, CA, USA; 2: University of Central Florida, College of Engineering and Computer Science, FL, USA; 3: University of California Los Angeles, Shool of Dentistry, CA, USA; 4: University of Washington, School of Dentistry, WA, USA


A Novel Approach for Selective Enrichment of Custom Gene Targets for Oncology Research

Bjoern Textor1, Andrew Barry2, Daniel Kraushaar3, Sarah Bowman3, Lynne Apone2, Kruti Patel3, Noa Henig3, Amy Emerman3, Theodore Davis2, Salvaltore Russello2, Cynthia Hendrickson3

1: New England Biolabs GmbH, Germany; 2: New England Biolabs Inc., U.S.; 3: Directed Genomics Inc., U.S.


Liquid biopsies, biomarker signatures, and beyond - why standardization of small RNA-Seq matters

Dominik Buschmann1,2, Anna Haberberger1, Benedikt Kirchner1, Melanie Spornraft1, Irmgard Riedmaier4,5, Gustav Schelling3, Michael W. Pfaffl1

1: Department of Animal Physiology and Immunology, TUM School of Life Sciences Weihenstephan, Technical University of Munich, Freising, Germany; 2: Institute of Human Genetics, University Hospital, Ludwig-Maximilians-University Munich, Germany; 3: Department of Anesthesiology, University Hospital, Ludwig-Maximilians-University, Munich, Germany; 4: Department of Physiology, TUM School of Life Sciences Weihenstephan, Technical University of Munich, Freising, Germany; 5: Eurofins Medigenomix Forensik GmbH, Ebersberg, Germany


Single-tube library prep solutions for high quality DNA sequencing

Yi Jin1,2, Marissa Bolduc2, David Bays2, Shuhong Li2, Hongbo Liu2, David Schuster1,2

1: Quantabio, United States of America; 2: QIAGEN Beverly, United States of America


Forensic Application Of Microbiome Profiling

Lisa-Marie Link1,2, Jens Söchtig2, Irmgard Riedmaier-Sprenzel2, Burkhard Rolf2

1: Eurofins Medigenomix GmbH, Germany; 2: Eurofins Medigenomix Forensik GmbH, Germany


Challenges of targeted NGS analyses

David Langenberger

ecSeq GmbH, Germany

ncRNA: non-coding RNAs
Location: Lecture hall 15
Chair: Pieter Mestdagh, Ghent University, Belgium
Chair: Michael W Pfaffl, TUM, Germany
 

Cancer-specific long non-coding RNAs as novel biomarkers and targets for therapy.

Pieter Mestdagh

Ghent University, Belgium


The potentials of isomiRs -- a new dimension in biomarker research.

Benedikt Kirchner, Ming Wu, Dominik Buschmann, Michael W. Pfaffl

Animal Physiology & Immunology, TUM School of Life Sciences Weihenstephan, Technical University of Munich, Freising, Germany


Epitranscriptomic regulation of non-coding RNAs

Stefan Ludwig Ameres

IMBA, Austria


Extracellular Vesicles and Their Associated Cargos in Health and Disease.

Mirco Castoldi

University of Düsseldorf, Germany


In silico functional analysis of microRNA : Towards the characterisation of miRNAs as biomarkers.

Artemis Hatzigeorgiou

Univeristy of Thessaly, Greece


A novel assay system for improved specificity and sensitivity of miRNA detection for all the major model organisms

Simon Baker

Bioline Reagents Limited, United Kingdom


Two-tailed RT-qPCR: a Novel Method for Highly Accurate miRNA Quantification

Lukas Valihrach1, Peter Androvic1, Julie Elling2, Robert Sjoback2, Mikael Kubista1,2

1: Institute of Biotechnology AS CR, Czech Republic; 2: TATAA Biocenter AB, Sweden

7:00pm
-
11:59pm
Bräustüberl: Symposium Dinner & Party @ Bräustüberl
Location: Bräustüberl Weihenstephan
Date: Wednesday, 05/Apr/2017
9:00am
-
12:30pm
MIQE&QC: MIQE & qPCR Quality Control
Location: Lecture hall 14
Chair: Stephen Andrew Bustin, Anglia Ruskin University, United Kingdom
Chair: Jan M Ruijter, Academic Medical Center, Netherlands, The
 

Why Are We Still Using qPCR For The Quantification Of Nucleic Acids?

Stephen Andrew Bustin

Anglia Ruskin University, United Kingdom


Target Concentration and Replicate qPCR Reactions

Jan M Ruijter1, Maurice WJ de Ronde2, Antoni Bayes Genis3, Yigal Pinto4, Sara-Joan Pinto2

1: Anatomy, Embryology and Physiology, Academic Medical Center, Amsterdam, the Netherlands; 2: Vascular Medicine & Clinical Epidemiology, Biostatistics and Bioinformatics, Academic Medical Center, Amsterdam, the Netherlands; 3: Heart Failure Unit, Germans Trias i Pujol Hospital, Universitat Autònoma, Barcelona, Spain; 4: Experimental Cardiology, Academic Medical Center, Amsterdam, the Netherlands


Laboratory Automation And Data Management In Diagnostics – qPCR ZIKV Detection And Quantification

Klemen Zupancic1, Misa Korva2, Tatjana Avsic Zupanc2, Urska Cepin3, Manca Pirc3, Laura Simdon4, Matjaz Hren1,3

1: SCINOTE, LLC, United States of America; 2: Institute of Microbiology and Immunology, Faculty of Medicine, University of Ljubljana, Slovenia; 3: BioSistemika, d.o.o., Slovenia; 4: Gilson, Inc., United States of America


Validation of a qPCR method - Determining Limit of Detection, Limit of Quantification and Dynamic Range.

Mikael Kubista1, Robert Sjöback1, Jens Björkman1, Björn Sjögreen2, Lucas Luke Linz3, Amin Forootan2

1: TATAA Biocenter AB, Sweden; 2: MultiD Analyses AB, Sweden; 3: LGC Douglas Scientific USA


Influence of PCR consumables on the accuracy of real-time PCR experiments and NGS sample preparation

Gerrit Gutzke, Hanna Oldfield, Emily Flowers

4titude Ltd, Germany


How to monitor analytical and technical factors influencing qPCR

Jens Bjorkman

TATAA Biocenter, Sweden

dPCR 2: digital PCR 2
Location: Lecture hall 15
Chair: Afif M. Abdel Nour, Holy Spirit University of Kaslik, Lebanon (Lebanese Republic)
Chair: David Svec, TATAA Biocenter, Czech Republic
 

Droplet Digital PCR for MYD88L265P Mutation Detection in Waldenström Macroglobulinemia: Minimal Residual Disease Monitoring and Characterization on Circulating Free DNA

Daniela Drandi1, Elisa Genuardi1, Irene Dogliotti1, Martina Ferrante1, Cristina Jimenez2, Francesca Guerrini3, Mariella Lo Schirico1, Vittorio Muccio1, Barbara Mantoan1, Milena Gilestro4, Paola Omedè4, Sara Galimberti3, Lorella Orsucci4, Federica Cavallo1, Ramon Garcia Sanz2, Mario Boccadoro1,4, Marco Ladetto5, Simone Ferrero1

1: Department of Molecular Biotechnologies and health sciences, Hematology Division, University of Torino, Italy; 2: Servicio de Hematología, Hospital Universitario de Salamanca, Salamanca, Espania; 3: Clinical and Experimental Medicine, University of Pisa, Pisa, Italy; 4: Division of Hematology, ASO S.Giovanni Battista, Torino, Italy; 5: Division of Hematology, Az Ospedaliera SS Antonio e Biagio e Cesare Arrigo, Alessandria, Italy


Partition Volume in dPCR -- Monodispersity Not Really Important

Joel Tellinghuisen

Vanderbilt University, United States of America


Automated cell picking and single cell digital PCR focusing on mitochondrial transfer

David Svec1,2

1: Institute of Biotechnology AS CR, Prague; 2: TATAA Biocenter, Czech Republic


Digital PCR Inhibition Mechanisms Using Standardized Inhibitors Representing Soil And Blood Matrices

Maja Sidstedt1,2, Erica L. Romsos3, Ronny Hedell2,4, Carolyn R. Steffen3, Peter M. Vallone3, Peter Rådström1, Johannes Hedman1,2

1: Applied Microbiology, Department of Chemistry, Lund University, PO Box 124, 221 00 Lund, Sweden; 2: Swedish National Forensic Centre, 581 94 Linköping, Sweden; 3: Materials Measurement Laboratory, National Institute of Standards and Technology, Gaithersburg, Maryland 20899-8314, United States; 4: Department of Mathematical Sciences, Chalmers University of Technology and University of Gothenburg, 41296 Gothenburg, Sweden


dpcReport: web server and software suite for unified analysis of digital PCRs and digital assays

Michał Burdukiewicz1, Jim Hugget2, Alexandra Whale2, Boris Fehse3, Piotr Sobczyk4, Paweł Mackiewicz1, Andrej-Nikolai Spiess5, Peter Schierack6, Stefan Rödiger6

1: Department of Genomics, University of Wrocław, Poland; 2: Molecular and Cell Biology Team, LGC, Teddington, United Kingdom; 3: Research Dept. Cell and Gene Therapy, Department of SCT, University Medical Center Hamburg-Eppendorf, Hamburg, Germany; 4: Faculty of Pure and Applied Mathematics, Wrocław University of Science and Technology, Wrocław, Poland; 5: University Medical Center Hamburg-Eppendorf, Hamburg, Germany; 6: Institute of Biotechnology, Brandenburg University of Technology Cottbus-Senftenberg, Senftenberg, Germany


A Statistical Contribution to the Uncertainty of Concentration Measurements Using Digital PCR

Andreas Kummrow, Annabell Plauth, Martin Hussels

Physikalisch-Technische Bundesanstalt, Germany

12:30pm
-
1:30pm
LTS-Kramer: Technical Lunch-time Seminar by F Kramer
Location: Lecture hall 14
Chair: Fred Russell Kramer, Rutgers University, United States of America
 

Multiplex Real-Time PCR Assays that measure the Abundance of Extremely Rare Mutations Associated with Cancer

Fred Russell Kramer

Rutgers University, United States of America

BDQ Editor Meeting
Location: Lecture hall 15
Chair: Stephen Andrew Bustin, Anglia Ruskin University, United Kingdom
Chair: Justin O'Grady, University of East Anglia, United Kingdom
12:30pm
-
2:00pm
PO3: Postersession 3
Location: Foyer - lower level
All posters will be displayed in all three poster sessions (Monday evening and two lunch poster sessions). Poster setup is on Monday afternoon and all posters are available until Wednesday afternoon.
Lunch Wednesday
Location: University Mensa
1:45pm
-
2:00pm
BDQ Poster Award
Location: Lecture hall 15
Chair: Jim Huggett, LGC & University of Surrey, United Kingdom
Chair: Lynn Sherrer, Elsevier, United States of America
2:00pm
-
4:30pm
DA: qPCR Data Analysis
Location: Lecture hall 14
Chair: Stefan Rödiger, BTU Cottbus - Senftenberg, Germany
Chair: Andrej-Nikolai Spiess, University Hospital Hamburg-Eppendorf, Germany
 

Methods and Technologies for Analysis Digital PCR Experiments - Lessons We Learned from the qPCR Technology

Stefan Rödiger1, Andrej-Nikolai Spiess2, Michał Burdukiewicz3

1: Brandenburg University of Technology Cottbus–Senftenberg, Senftenberg, Germany; 2: University Medical Center Hamburg-Eppendorf, Hamburg, Germany; 3: University of Wroclaw, Wroclaw, Poland


Development of an HRM-Based Tool for the Automated Identification of Nucleotide Sequences in Large Datasets

Jean-Christophe Avarre1, Matthieu Vignoles2, Mathieu Laffont2, Lise Grewis2, Christelle Reynes3,4

1: Institut des Sciences de l'Evolution de Montpellier, UMR IRD-CNRS-UM-EPHE, Montpellier, France; 2: IDVet Genetics, Montpellier, France; 3: Institute of Functional Genomics, UMR CNRS-INSERM-UM, Montpellier, France; 4: Laboratory of Biostatistics, Informatics and Pharmaceutical Physics, UFR Pharmacy, University of Montpellier, France


A New Paradigm in Genetic Data Analysis on the Thermo Fisher Cloud

Nivedita Majumdar, Puneet Suri, Gloria Lam, David Woo, Shakila Pothini

Thermofisher Scientific, United States of America


COMplementary Primer ASymmetric PCR (COMPAS™ -PCR), A Counter Intuitive Primer Design For PCR

Marc Anglès d'Auriac

Norwegian Institute for Water Research, Norway


Comparative Procedures for Sample Processing and Development of qPCR Assays for the Rapid Detection of Grapevine and Citrus Pathogens

Emir Hodzic

School of Veterinary Medicine, University of California at Davis, United States of America

MG: MicroGenomics & Single-Cell-qPCR
Location: Lecture hall 15
Chair: Mikael Kubista, TATAA Biocenter AB, Sweden
Chair: Anders Ståhlberg, University of Gothenburg, Sweden
 

Computational Analysis of Single-Cell RNA-Seq Profiles Identifies Lineage Choice and Graded Transitions in Myeloid Progenitors

Fabian J. Theis

Helmholtz Zentrum München/ TUM, Germany


High Resolution Single Cell Analysis In Complex Adult Tissues

Stefan Günther, Michail Yekelchyk, Isabelle Salwig, Jens Preussner, Thomas Braun

MPI for Heart and Lung Research, Germany


A Cost Effective 5’ Selective Single Cell Transcriptome Profiling Approach

Pascal Barbry1,2, Marie-Jeanne Arguel1,2, Kévin Lebrigand1,2, Agnès Paquet1,2, Sandra Ruiz-Garcia1,2, Laure-Emmanuelle Zaragosi1,2, Rainer Waldmann1,2

1: Université Côte d'Azur; 2: CNRS


Deeper Understanding Starts With a Single Cell

Afif M. Abdel Nour1, Georges Nemer2, Lara Hanna Wakim1, Esam Azhar3

1: Faculty of Agricultural and Food Sciences, Holy Spirit University of Kaslik, Jounieh, Lebanon; 2: Department of Biochemistry and Molecular Genetics, American University of Beirut-Medical Center, Beirut, Lebanon; 3: Special Infectious Agents Unit-Biosafety Level 3, King Fahd Medical Research Center, King Abdulaziz University, Jeddah, Kingdom of Saudi Arabia, Medical Laboratory Technology Department, Faculty of Applied Medical Sciences, King Abdulaziz University, Jeddah, Kingdom of Saudi Arabia


Whole transcriptome subcellular profiling of the Xenopus oocyte.

Radek Sindelka, Pavel Abaffy, Silvie Tomankova, Ravindra Naraine, Mikael Kubista

Institute of Biotechnology-Biocev, Czech Republic

4:30pm
-
5:00pm
Farewell
Location: Lecture hall 14
Chair: Michael W Pfaffl, TUM, Germany
Chair: Sylvia Pfaffl, bioMCC, Germany
Date: Thursday, 06/Apr/2017
9:00am
-
5:00pm
WS1: Workshops Day 1
Location: Workshop Rooms
Date: Friday, 07/Apr/2017
9:00am
-
5:00pm
WS2: Workshops Day 2
Location: Workshop Rooms

 
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Conference: qPCR dPCR & NGS 2017
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