Overview and details of the sessions of this conference. Please select a date or location to show only sessions at that day or location. Please select a single session for detailed view (with abstracts and downloads if available).
LTS-Kramer: Technical Lunch-time Seminar by F Kramer
12:30pm - 1:30pm
Session Chair: Fred Russell Kramer, Rutgers University, United States of America
Location:Lecture hall 14 650 participants, TUM Weihenstephan
Multiplex Real-Time PCR Assays that measure the Abundance of Extremely Rare Mutations Associated with Cancer
Fred Russell Kramer
Rutgers University, United States of America
PCR assays are the most rapid, most sensitive, and least expensive way to assess the abundance of mutant DNA fragments present in liquid biopsies. “SuperSelective” PCR primers, due to their unique design, are extraordinarily specific, able to selectively initiate the synthesis of amplicons on ten mutant DNA fragments in the presence of 1,000,000 wild-type DNA fragments. Sets of SuperSelective primers, each possessing unique 5’-tag sequences, enable the amplicons generated from each mutant to be distinguished by differently colored molecular beacon probes. And the inclusion of primers for a wild-type reference gene fragment, enables the abundance of each type of mutant DNA fragment to be assessed by determining the difference between its threshold value and the threshold value of the reference gene.