Conference Agenda

STATE-DEPENDENT KCNQ1 INHIBITOR BREAKS C4 SYMMETRY TO BLOCK ION CONDUCTION

Efthimios Kyriakis¹, Agnese Roscioni², Jodene Eldstrom¹, Luca Maragliano², Filip Van Petegem¹, David Fedida¹

¹University of British Columbia, Canada; ²Polytechnic University of Marche, Via Brecce Bianche, Ancona 60131, Italy

CONFORMATIONS OF KV7.1 PORE MORPH WITH VOLTAGE SENSOR STATES AND REGULATORY SUBUNITS

Lei Huang¹, Nitzan Daus², Yuyin Wang¹, Guohui Zhang¹, Manar Khier², Ariel Ben-Bassat², Jingyi Shi¹, Lu Zhao¹, Borui Zhang¹, Lu Han¹, Yoni Haitin², Bernard Attali², Jianmin Cui¹

¹Washington University in St. Louis, USA, United States of America; ²Tel Aviv University, Tel Aviv, Israel

STRUCTURE AND PHOSPHORYLATION MECHANISMS OF THE CARDIAC KCNQ1+KCNE1 CHANNEL UNDER STRESS CONDITION

Ling Zhong¹, Zhenzhen Yan¹, Xiaoqing Lin¹, Xinyu Cheng², Shuangyan Wan², Jin Zhang², Panpan Hou¹

¹Macau University Of Science And Technology, Macau S.A.R. (China); ²Nanchang University, China

CHEMICAL TUNING REVEALS A CATION-PI GATING BRIDGE BETWEEN THE VOLTAGE-SENSOR AND PORE DOMAINS IN THE KV7.1 POTASSIUM CHANNEL

Christopher Ahern, Miranda Schene

University of Iowa, United States of America

FUNCTIONAL CHARACTERIZATION OF KV7.2/7.3, KV7.2/7.5, AND KV7.3/7.5 CHANNELS: IMPACT OF SUBUNIT COMPOSITION AND PHARMACOLOGICAL MODULATION

Vivian Meiritz¹, Guiscrad Seebohm², Thomas Budde¹

¹Institute of Physiology I, University of Münster, Münster, Germany; ²Department of Cardiovascular Medicine, Institute for Genetics of Heart Diseases (IfGH), University Hospital Münster, Münster, Germany

AT THE INTERSECTION BETWEEN SODIUM AND POTASSIUM CHANNELS: M-CURRENT DOWNREGULATION DRIVES CORTICAL HYPEREXCITABILITY IN THE SODIUM CHANNELOPATHY DRAVET SYNDROME

Stefano Iavarone¹, Peter Müller-Wöhrstein¹, Oleg Vinogradov¹, Edueni Erharhaghen¹, Nikolas Layer^1,2, Albina Farkhutidnova¹, Francesco Miceli³, Maurizio Taglialatela³, Carmine Ostacolo⁴, Henner Koch⁵, Holger Lerche¹, Thomas V. Wuttke^1,6, Ulrike B.S. Hedrich¹

¹Hertie Institute for Clinical Brain Research, Germany; ²Neurology Clinic and National Center for Tumor Diseases, University Hospital Heidelberg, Heidelberg, Germany; Department of Functional Neuroanatomy, Institute for Anatomy and Cell Biology, Heidelberg University, Heidelberg, Germany.; ³Department of Neuroscience, University of Naples "Federico II", Naples, Italy; ⁴Department of Pharmacy, Medicinal Chemistry Section, University of Salerno, Italy; ⁵Department of Epileptology, Neurology, Rheinisch-Westfälische Technische Hochschule Aachen, Aachen, Germany.; ⁶Department of Neurosurgery, University of Tübingen, Tübingen, Germany

PREDICTING NEURODEVELOPMENTAL OUTCOME IN KCNQ2-RELATED DISORDERS

Elisabeth Van Boxstael¹, Charissa Millevert¹, Megan Hairabedian², Dennis Lal³, Mathieu Milh⁴, Sarah Weckhuysen¹

¹Translational Epilepsy Genomics Group, VIB Center for Molecular Neurology, University of Antwerp, Antwerp, Belgium.; ²Pediatric Neurology Department, Pediatric Hospitals of Nice, Lenval University Hospital Center, Nice, France; ³Department of Neurology, McGovern Medical School, University of Texas Health Science Center, Houston, Texas, United States; ⁴Department of Pediatric Neurology, Timone Children’s Hospital, Marseille, France

A GATING NEXUS BETWEEN THE S6-HELIX A AND HELIX C DOMAINS STABILIZES THE ACTIVE CONFORMATION OF KV7 CHANNELS

Shawn Lamothe, Thomas Hammond, Harley Kurata

University of Alberta, Canada

RESOLVING CA²⁺-DEPENDENT CALMODULIN REGULATORY STATES OF KV7.2 USING A FRET-BASED APPROACH

Emily Walsh, Phuc Phan, Crystal Archer

University of Arkansas, United States of America

MOLECULAR DISSECTION OF THE KV7.2-STARGAZIN INTERACTION

Telmo Leal^1,2, Marina Rodrigues¹, Ângela Inácio¹, Raquel Gouveia^1,2, Ilaria Mosca³, Paolo Ambrosino³, Maria Virginia Soldovieri³, Irina Moreira^1,4, Maurizio Taglialatela⁵, Ana Luísa Carvalho^1,4,6

¹CNC - Center for Neuroscience and Cell Biology, University of Coimbra, 3004-504 Coimbra, Portugal; ²PDBEB – Doctoral Program in Biomedicine and Molecular Biology, University of Coimbra; ³Department of Medicine and Health Science Vincenzo Tiberio, University of Molise, 86100 Campobasso; ⁴CiBB - Centre for Innovative Biomedicine and Biotechnology, University of Coimbra; ⁵Department of Neuroscience, Reproductive Sciences and Dentistry, University Federico II of Naples, 80131 Naples, Italy; ⁶DCV-UC -Department of Life Sciences, University of Coimbra, 3000-456 Coimbra, Portugal.

ATOMISTIC INSIGHTS INTO KV7.2 VARIANT-INDUCED DYSFUNCTION: A MOLECULAR DYNAMICS STUDY

Agnese Roscioni¹, Giulio Alberini^2,3, Lorenzo Cornice Durante¹, Francesco Miceli⁴, Maurizio Taglialatela⁴, Luca Maragliano^1,2

¹Polytechnic University of Marche, Ancona, Italy; ²Center for Synaptic Neuroscience and Technology, Istituto Italiano di Tecnologia, Genova, Italy; ³IRCCS Ospedale Policlinico San Martino, Genova, Italy; ⁴University of Naples “Federico II”, 80131 Naples, Italy

MECHANISTIC CHARACTERIZATION AND THERAPEUTIC TARGETING OF KCNQ2 DEVELOPMENTAL AND EPILEPTIC ENCEPHALOPATHY IN A KV7.2 THR274MET/+ MOUSE MODEL

Shaimaa Haiba^1,7, Kilian Lüdicke^1,8, Laurent Villard^2,3, Maurizio Taglialatela⁴, Carmine Ostacolo⁵, Henner Koch⁶, Nelly Timm⁶, Holger Lerche¹, Thomas V. Wuttke^1,8

¹Department of Neurology and Epileptology, Hertie Institute for Clinical Brain Research, Tübingen, Germany; ²Aix-Marseille University, INSERM, Marseille Medical Genetics Centre (MMG), Marseille, France; ³Department of Medical Genetics, La Timone Children’s Hospital, Marseille, France; ⁴Department of Neuroscience, Reproductive Sciences and Dentistry, University Federico II of Naples, Italy; ⁵Department of Pharmacy, University of Salerno, Italy; ⁶Department of Neurology, Section Epileptology, Aachen, Germany; ⁷International Max Planck Research School for the Mechanisms of Mental Function and Dysfunction, Tübingen, Germany; ⁸Department of Neurosurgery, Tübingen, Germany

EFFECTS OF KV7 CHANNEL ACTIVATORS IN EXPERIMENTAL MODELS OF AMYOTROPHIC LATERAL SCLEROSIS

Rossella Vitale^1,2, Camilla Celentano¹, Flavia Borghese¹, Lidia Carotenuto¹, Luana Messuri¹, Natascia Guida¹, Carmine Ostacolo³, Francesco Miceli¹, Maurizio Taglialatela¹, Vincenzo Barrese¹

¹Department of Neuroscience, Reproductive Sciences and Dentistry, University of Naples, Federico II, Naples, Italy; ²International School of Advanced Studies, University of Camerino, Camerino, Italy; ³Department of Pharmacy, University of Salerno, Salerno, Italy

MODELING KCNQ2-ENCEPHALOPATHY USING IPSC-DERIVED EXCITATORY-INHIBITORY NEURONAL NETWORKS

Noortje Zonnekein^1,2,3, Lidia Carotenuto^1,3,4,5, Yara Meijs^1,2, Marcus Kaji^1,3,4,5, Nina Dirkx^1,2, Els De Vriendt¹, Peter Verstraelen⁶, Simona Manzella^1,2, Bob Asselbergh^1,2, Geert Joris^1,2, Daphné Deben^1,2, Lamyae Elangouri^1,2, Karlijn Cuypers^1,2, Sarah Weckhuysen^1,3,4,5

¹VIB-UAntwerp, Belgium; ²Department of Biomedical Sciences, University of Antwerp, Antwerp, Belgium.; ³μNeuro Research Centre of Excellence, University of Antwerp, Antwerp, Belgium; ⁴Division of Neurology, University Hospital Antwerp, Antwerp, Belgium.; ⁵Translational Neurosciences, Faculty of Medicine and Health Science, University of Antwerp, Antwerp, Belgium.; ⁶Laboratory of Cell Biology and Histology, University of Antwerp, Antwerp, Belgium.

ENDOTHELIAL KV7.5 CHANNELS REGULATE BLOOD–BRAIN BARRIER INTEGRITY AND SEIZURE SUSCEPTIBILITY

Camilla Celentano^1,2, Ryan F Yoshimura², Francesco Miceli¹, Maurizio Taglialatela¹, Geoffrey W Abbott², Vincenzo Barrese¹

¹Department of Neuroscience, Reproductive Sciences and Dentistry, University of Naples Federico II, Naples, Italy; ²Bioelectricity Laboratory, Department of Physiology and Biophysics, School of Medicine, University of California, Irvine, CA 92697

REGULATION OF VASCULAR KV7 CHANNELS BY G PROTEIN‑COUPLED RECEPTOR KINASES

Skye Lau, Miguel Benítez-Angeles, Iain Greenwood

School of Health and Medical Sciences, City St George’s, University of London, London