Session | ||
MS-42: Novel techniques and insights into in vitro and in situ crystallisation for X-ray and electron diffraction.
Invited: Haruki Hasegawa (USA), Alexandra Ros (USA) | ||
Session Abstract | ||
This microsymposium will aim to present challenges and opportunities in structure determination by crystallography posed by nano, micro and giant crystals. These include new insights into the process of crystallisation and methodological advances that facilitate the production, manipulation and analysis of biological macromolecule crystals, particularly for serial diffraction experiments at microfocus synchrotron and free-electron laser sources, but also for new applications of electron diffraction. For all abstracts of the session as prepared for Acta Crystallographica see PDF in Introduction, or individual abstracts below. | ||
Introduction | ||
Presentations | ||
10:20am - 10:25am
Introduction to session 10:25am - 10:55am
Concurrent crystallization of multiple proteins in a single cell without interfering each other’s phase separation events Amgen Inc, South San Francisco, United States of America External Resource: https://www.xray.cz/iucrv/vidp.asp?id=313
10:55am - 11:25am
Microfluidic Tools Reducing Sample Amount in Serial Crystallography with XFELs Arizona State University, Tempe, United States of America External Resource: https://www.xray.cz/iucrv/vidp.asp?id=314
11:25am - 11:45am
MyD88 TIR domain higher-order assembly interactions revealed by microcrystal electron diffraction and serial femtosecond crystallography 1Australian Research Council Centre of Excellence in Advanced Molecular Imaging, Department of Chemistry and Physics, La Trobe Institute for Molecular Science, La Trobe University, Melbourne, Victoria, Australia; 2Department of Materials and Environmental Chemistry, Stockholm University, Stockholm, Sweden; 3School of Chemistry and Molecular Biosciences, The University of Queensland, Brisbane, Queensland, Australia; 4Department of Biological Chemistry, University of California Los Angeles, Los Angeles, California, USA; 5Institute for Glycomics, Griffith University, Southport, Queensland, Australia.; 6EMBL Australia Node in Single Molecule Science, University of New South Wales, Kensington, New South Wales, Australia; 7Institute for Molecular Bioscience, The University of Queensland, Brisbane, Queensland, Australia; 8Cambridge Institute for Medical Research, University of Cambridge, Cambridge, UK.; 9Linac Coherent Light Source, SLAC National Accelerator Laboratory, Menlo Park, California, USA.; 10Australian Infectious Diseases Research Centre, The University of Queensland, Brisbane, Queensland, Australia. External Resource: https://www.xray.cz/iucrv/vidp.asp?id=315
11:45am - 12:05pm
The crystallomics pipeline, a shotgun approach on native proteomes to (re)discover the unsuspected 1Swiss Light Source, Paul Scherrer Institut, Forschungsstrasse 111, Villigen-PSI, 5232, Switzerland; 2European Synchrotron Radiation Facility, 38043 Grenoble, France; 3Max-Planck-Institut für Marine Mikrobiologie, Celsiusstraße 1, 28359, Bremen, Germany; 4Structural Biology Research Center, Photon Factory, Institute of Materials Structure Science, High Energy Accelerator Research Organization, Tsukuba, 305-0801, Japan.; 5Institut de Biologie Structurale (IBS), Universite ́ Grenoble Alpes, CEA, CNRS, 71 avenue des Martyrs, Grenoble Cedex 9, 38044, France External Resource: https://www.xray.cz/iucrv/vidp.asp?id=316
12:05pm - 12:25pm
Protein crystallization assisted by the crystallophore. 1Institut de Biologie Structurale, Grenoble, France; 2Ecole Normale Supérieure, Lyon, France External Resource: https://www.xray.cz/iucrv/vidp.asp?id=317
12:25pm - 12:45pm
Protein crystallisation in agarose gel, a cheap and versatile technique 1CSIC, Granada, Spain; 2Politecnico di Torino, Torino, Italy; 3Structural Biology, Molecular Biology Institute of Barcelona, Barcelona, Spain External Resource: https://www.xray.cz/iucrv/vidp.asp?id=318
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