Conference Agenda

Overview and details of the sessions of this conference. Please select a date or location to show only sessions at that day or location. Please select a single session for detailed view (with abstracts and downloads if available).

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Session Overview
MS-42: Novel techniques and insights into in vitro and in situ crystallisation for X-ray and electron diffraction.
Wednesday, 18/Aug/2021:
10:20am - 12:45pm

Session Chair: Lars Redecke
Session Chair: Fasseli Coulibaly
Location: Club A

170 1st floor

Invited: Haruki Hasegawa (USA), Alexandra Ros (USA)

Session Abstract

This microsymposium will aim to present challenges and opportunities in structure determination by crystallography posed by nano, micro and giant crystals. These include new insights into the process of crystallisation and methodological advances that facilitate the production, manipulation and analysis of biological macromolecule crystals, particularly for serial diffraction experiments at microfocus synchrotron and free-electron laser sources, but also for new applications of electron diffraction.

For all abstracts of the session as prepared for Acta Crystallographica see PDF in Introduction, or individual abstracts below.

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10:20am - 10:25am

Introduction to session

Lars Redecke, Fasseli Coulibaly

10:25am - 10:55am

Concurrent crystallization of multiple proteins in a single cell without interfering each other’s phase separation events

Haruki Hasegawa

Amgen Inc, South San Francisco, United States of America

10:55am - 11:25am

Microfluidic Tools Reducing Sample Amount in Serial Crystallography with XFELs

Alexandra Ros

Arizona State University, Tempe, United States of America

11:25am - 11:45am

MyD88 TIR domain higher-order assembly interactions revealed by microcrystal electron diffraction and serial femtosecond crystallography

Max T. B. Clabbers2,4, Susannah Holmes1, Timothy W. Muusse3, Parimala Vajjhala3, Sara J. Thygesen3, Alpeshkumar K. Malde5, Dominic J. B. Hunter3,6,7, Tristan I. Croll8, Leonie Flueckiger1, Jeffrey D. Nanson3, Md. Habibur Rahaman3, Andrew Aquila9, Mark S. Hunter9, Mengning Liang9, Chun Hong Yoon9, Jingjing Zhao2, Nadia A. Zatsepin1, Brian Abbey1, Emma Sierecki6, Yann Gambin6, Katryn J. Stacey3, Connie Darmanin1, Bostjan Kobe3,7,10, Hongyi Xu2, Thomas Ve5

1Australian Research Council Centre of Excellence in Advanced Molecular Imaging, Department of Chemistry and Physics, La Trobe Institute for Molecular Science, La Trobe University, Melbourne, Victoria, Australia; 2Department of Materials and Environmental Chemistry, Stockholm University, Stockholm, Sweden; 3School of Chemistry and Molecular Biosciences, The University of Queensland, Brisbane, Queensland, Australia; 4Department of Biological Chemistry, University of California Los Angeles, Los Angeles, California, USA; 5Institute for Glycomics, Griffith University, Southport, Queensland, Australia.; 6EMBL Australia Node in Single Molecule Science, University of New South Wales, Kensington, New South Wales, Australia; 7Institute for Molecular Bioscience, The University of Queensland, Brisbane, Queensland, Australia; 8Cambridge Institute for Medical Research, University of Cambridge, Cambridge, UK.; 9Linac Coherent Light Source, SLAC National Accelerator Laboratory, Menlo Park, California, USA.; 10Australian Infectious Diseases Research Centre, The University of Queensland, Brisbane, Queensland, Australia.

11:45am - 12:05pm

The crystallomics pipeline, a shotgun approach on native proteomes to (re)discover the unsuspected

Sylvain Engilberge1,2, Olivier Lemaire3, Marie-Caroline Mueller3, Filip Leonarski3, Chia-Ying Huang1, Takashi Tomizaki1, Naohiro Matsugaki5, Antoine Royant4, Vincent Olieric1, Meitian Wang1, Tristan Wagner3

1Swiss Light Source, Paul Scherrer Institut, Forschungsstrasse 111, Villigen-PSI, 5232, Switzerland; 2European Synchrotron Radiation Facility, 38043 Grenoble, France; 3Max-Planck-Institut für Marine Mikrobiologie, Celsiusstraße 1, 28359, Bremen, Germany; 4Structural Biology Research Center, Photon Factory, Institute of Materials Structure Science, High Energy Accelerator Research Organization, Tsukuba, 305-0801, Japan.; 5Institut de Biologie Structurale (IBS), Universite ́ Grenoble Alpes, CEA, CNRS, 71 avenue des Martyrs, Grenoble Cedex 9, 38044, France

12:05pm - 12:25pm

Protein crystallization assisted by the crystallophore.

Eric Girard1, Zaynab Alsalman1, Adeline Robin1, Sylvain Engilberge1, Amandine Roux2, François Riobé2, Olivier Maury2

1Institut de Biologie Structurale, Grenoble, France; 2Ecole Normale Supérieure, Lyon, France

12:25pm - 12:45pm

Protein crystallisation in agarose gel, a cheap and versatile technique

Jose A. Gavira1, Fiora Artusio2, Albert Castellví3, Roberto Pisano2

1CSIC, Granada, Spain; 2Politecnico di Torino, Torino, Italy; 3Structural Biology, Molecular Biology Institute of Barcelona, Barcelona, Spain

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