Conference Agenda

Overview and details of the sessions of this conference. Please select a date or location to show only sessions at that day or location. Please select a single session for detailed view (with abstracts and downloads if available).

Please note that all times are shown in the time zone of the conference. The current conference time is: 1st Nov 2024, 12:48:08am CET

 
 
Session Overview
Session
MS-33: CryoEM for macromolecules - from single particles to microcrystals
Time:
Tuesday, 17/Aug/2021:
10:20am - 12:45pm

Session Chair: Jan Pieter Abrahams
Session Chair: Jiri Novacek
Location: Club A

170 1st floor

Invited:  Nenad Ban (Switzerland), Dimple Karia (Netherlands)


Session Abstract

Cryogenic electron microscopy (cryo-EM) rivals X-ray crystallography in terms of resolution and can be used to determine atomic structures of macromolecules that are either refractory to crystallization or difficult to crystallize in specific functional states. Electron diffraction can yield structures of proteins even when crystals are smaller than the wavelength of light. The session focuses on examples of both methods.  We encourage people working on both single particle cryo-EM of biological macromolecules and electron diffractions of microcrystals to submit their abstract and to attend the Microsymposium entitled “Cryo-EM for macromolecules – from single particles to microcrystals”.


For all abstracts of the session as prepared for Acta Crystallographica see PDF in Introduction, or individual abstracts below.

 


Introduction
Show help for 'Increase or decrease the abstract text size'
Presentations
10:20am - 10:25am
ID: 1763 / MS-33: 1
Introduction
Oral/poster

Introduction to session

Jan Pieter Abrahams, Jiří Nováček



10:25am - 10:55am
ID: 1418 / MS-33: 2
Biological and macromolecular crystallography
Invited lecture to session
MS: CryoEM for macromolecules - from single particles to microcrystals
Keywords: cryo-electron microscopy, protein synthesis, viral translation

Structural basis of SARS-CoV-2 translational shutdown and programmed ribosomal frameshifting

Nenad Ban

ETH Zurich, Zurich, Switzerland

External Resource:
Video Link


10:55am - 11:25am
ID: 906 / MS-33: 3
Biological and macromolecular crystallography
Invited lecture to session
MS: CryoEM for macromolecules - from single particles to microcrystals
Keywords: cryo-EM, Single Particle Analysis, Tundra, Glacios, GABAA receptor

Home source for cryo-EM

Dimple Karia1, Adrian Koh1, Zuzana Hlavenkova2, Milos Malinsky2, Vojtech Dolezal2, Alevtyna Yakushevska1, Lingbo Yu1, Abhay Kotecha1

1Thermo Fisher Scientific, Materials and Structural Analysis Division, Achtseweg Noord, Eindhoven, Netherlands; 2Thermo Fisher Scientific Brno s.r.o, Materials & Structural Analysis, Brno, Czech Republic

External Resource:
Video Link


11:25am - 11:50am
ID: 806 / MS-33: 4
Biological and macromolecular crystallography
Oral/poster
MS: CryoEM for macromolecules - from single particles to microcrystals, Structural biology of viruses - in memory of Michael Rossmann
Keywords: Flaviviruses, virus assembly, epidemic, cryo-EM, insect viruses

High-resolution structures of immature and chimeric flaviviruses reveal key features redefining viral architecture and maturation

Joshua Hardy1, Natalee Newton2, Naphak Modhiran2, Connor Scott2, Hari Venugopal1, Laura Vet2, Paul Young2, Roy Hall2, Jody Hobson-Peters2, Daniel Watterson2, Fasseli Coulibaly1

1Monash University, Clayton, VIC, Australia; 2The University of Queensland, Brisbane, QLD, Australia

External Resource:
Video Link


11:50am - 12:15pm
ID: 1190 / MS-33: 5
Biological and macromolecular crystallography
Oral/poster
MS: CryoEM for macromolecules - from single particles to microcrystals, Structural biology of viruses - in memory of Michael Rossmann, Structural biology of receptors, signaling and membrane proteins
Keywords: rhinovirus, ICAM-1, cryo-EM, single particle analysis, receptor

Receptor induced rearrangements of capsid and genome prime rhinovirus 14 for activation and uncoating

Dominik Hrebík, Tibor Füzik, Mária Gondová, Lenka Šmerdová, Athanassios Adamopoulos, Ondrej Šedo, Zbyněk Zdráhal, Pavel Plevka

CEITEC MU, Brno, Czech Republic

External Resource:
Video Link


12:15pm - 12:40pm
ID: 474 / MS-33: 6
Bursary application
Poster
MS: Structural bioinformatics, CryoEM for macromolecules - from single particles to microcrystals, Structural biology of viruses - in memory of Michael Rossmann, Structural biology of eukaryotic immune systems, Structural biology of receptors, signaling and membrane proteins, Protein design and engineering, Data-driven discovery in crystallography, Structural bioinformatics
Posters only: Hot structures of biological importance, Macromolecular and biological crystallography (if it does not fit to any specific topics)
Keywords: Keywords: Mycobacterium tuberculosis, Enolase, X-ray crystallography, CryoEM, Molecular dynamics

M. tuberculosis enolase: Crystal and Cryo-EM structures provide insights into 2-phosphoglycerate binding and catalysis

Mohammed Ahmad1, Bhavya Jha1, Sucharita Bose2, Satish Tiwari1, Abhisek Dwivedy1, Richard Mariadasse3, Ravikant Pal1, Tanya Parish4, J jayekanthan3, Vinothkumar kutti Ragunath2, Bichitra Kumar Biswal1

1National Institute of Immunology, New Delhi, India; 2National Centre for Biological Sciences, Bangalore 560065, India; 3Alagappa University ,Karaikudi, Tamil Nadu 630003, India; 4Infectious Disease Research Institute, 1616 Eastlake Avenue E, Suite 400, Seattle,WA98102, United States

Bibliography
Dwivedy A, Jha B, Singh KH, Ahmad M, Ashraf A, Kumar D, Biswal BK. (2018). Serendipitous crystallization and structure determination of bacterioferritin from Achromobacter. Acta Crystallogr Sect F Struct Biol Commun 74:558–566.

Mohammed Ahmad, Bhavya Jha, Satish Tiwari1, Abhisek Dwivedy,Richard Mariadasse, Ravikant Pal, Tanya Parish, J. jeyakanthan, Vinoth Kutti Ragunath and Bichitra Kumar Biswal, M. tuberculosis enolase: Crystal and Cryo-EM structures provide insights into 2-phosphoglycerate binding and catalysis (under review)


 
Contact and Legal Notice · Contact Address:
Privacy Statement · Conference: IUCr 2021 | August 14 - 22, 2021 | Prague, Czech Republic
Conference Software: ConfTool Pro 2.8.103+TC+CC
© 2001–2024 by Dr. H. Weinreich, Hamburg, Germany