XXV General Assembly and Congress of the International Union of Crystallography - IUCr 2021

Overview and details of the sessions of this conference. Please select a date or location to show only sessions at that day or location. Please select a single session for detailed view (with abstracts and downloads if available).

Session

MS-33: CryoEM for macromolecules - from single particles to microcrystals

Time:

Tuesday, 17/Aug/2021:

10:20am - 12:45pm

Session Chair: Jan Pieter Abrahams
Session Chair: Jiri Novacek

Location: Club A

170 1st floor

Invited: Nenad Ban (Switzerland), Dimple Karia (Netherlands)

Session Abstract

Cryogenic electron microscopy (cryo-EM) rivals X-ray crystallography in terms of resolution and can be used to determine atomic structures of macromolecules that are either refractory to crystallization or difficult to crystallize in specific functional states. Electron diffraction can yield structures of proteins even when crystals are smaller than the wavelength of light. The session focuses on examples of both methods. We encourage people working on both single particle cryo-EM of biological macromolecules and electron diffractions of microcrystals to submit their abstract and to attend the Microsymposium entitled “Cryo-EM for macromolecules – from single particles to microcrystals”.

For all abstracts of the session as prepared for Acta Crystallographica see PDF in Introduction, or individual abstracts below.

Introduction

Presentations

10:20am - 10:25am

Introduction to session

Jan Pieter Abrahams, Jiří Nováček

10:25am - 10:55am

Structural basis of SARS-CoV-2 translational shutdown and programmed ribosomal frameshifting

Nenad Ban

ETH Zurich, Zurich, Switzerland

External Resource:

10:55am - 11:25am

Home source for cryo-EM

Dimple Karia¹, Adrian Koh¹, Zuzana Hlavenkova², Milos Malinsky², Vojtech Dolezal², Alevtyna Yakushevska¹, Lingbo Yu¹, Abhay Kotecha¹

¹Thermo Fisher Scientific, Materials and Structural Analysis Division, Achtseweg Noord, Eindhoven, Netherlands; ²Thermo Fisher Scientific Brno s.r.o, Materials & Structural Analysis, Brno, Czech Republic

External Resource:

11:25am - 11:50am

High-resolution structures of immature and chimeric flaviviruses reveal key features redefining viral architecture and maturation

Joshua Hardy¹, Natalee Newton², Naphak Modhiran², Connor Scott², Hari Venugopal¹, Laura Vet², Paul Young², Roy Hall², Jody Hobson-Peters², Daniel Watterson², Fasseli Coulibaly¹

¹Monash University, Clayton, VIC, Australia; ²The University of Queensland, Brisbane, QLD, Australia

External Resource:

11:50am - 12:15pm

Receptor induced rearrangements of capsid and genome prime rhinovirus 14 for activation and uncoating

Dominik Hrebík, Tibor Füzik, Mária Gondová, Lenka Šmerdová, Athanassios Adamopoulos, Ondrej Šedo, Zbyněk Zdráhal, Pavel Plevka

CEITEC MU, Brno, Czech Republic

External Resource:

12:15pm - 12:40pm

M. tuberculosis enolase: Crystal and Cryo-EM structures provide insights into 2-phosphoglycerate binding and catalysis

Mohammed Ahmad¹, Bhavya Jha¹, Sucharita Bose², Satish Tiwari¹, Abhisek Dwivedy¹, Richard Mariadasse³, Ravikant Pal¹, Tanya Parish⁴, J jayekanthan³, Vinothkumar kutti Ragunath², Bichitra Kumar Biswal¹

¹National Institute of Immunology, New Delhi, India; ²National Centre for Biological Sciences, Bangalore 560065, India; ³Alagappa University ,Karaikudi, Tamil Nadu 630003, India; ⁴Infectious Disease Research Institute, 1616 Eastlake Avenue E, Suite 400, Seattle,WA98102, United States

Conference Agenda