XXV General Assembly and Congress of the
International Union of Crystallography - IUCr 2021
August 14 - 22, 2021 | Prague, Czech Republic
Conference Agenda
Overview and details of the sessions of this conference. Please select a date or location to show only sessions at that day or location. Please select a single session for detailed view (with abstracts and downloads if available).
Please note that all times are shown in the time zone of the conference. The current conference time is: 1st Nov 2024, 12:27:52am CET
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Session Overview |
Session | ||
MS-42: Novel techniques and insights into in vitro and in situ crystallisation for X-ray and electron diffraction.
Invited: Haruki Hasegawa (USA), Alexandra Ros (USA) | ||
Session Abstract | ||
This microsymposium will aim to present challenges and opportunities in structure determination by crystallography posed by nano, micro and giant crystals. These include new insights into the process of crystallisation and methodological advances that facilitate the production, manipulation and analysis of biological macromolecule crystals, particularly for serial diffraction experiments at microfocus synchrotron and free-electron laser sources, but also for new applications of electron diffraction. For all abstracts of the session as prepared for Acta Crystallographica see PDF in Introduction, or individual abstracts below. | ||
Introduction | ||
Presentations | ||
10:20am - 10:25am
ID: 1773 / MS-42: 1 Introduction Oral/poster Introduction to session 10:25am - 10:55am
ID: 380 / MS-42: 2 Biological and macromolecular crystallography Invited lecture to session MS: Novel techniques and insights into in vitro and in situ crystallisation for X-ray and electron diffraction Keywords: intracellular protein crystallization, phase separation, ER, cytosol, nucleus Concurrent crystallization of multiple proteins in a single cell without interfering each other’s phase separation events Amgen Inc, South San Francisco, United States of America 10:55am - 11:25am
ID: 1078 / MS-42: 3 Biological and macromolecular crystallography Invited lecture to session MS: Novel techniques and insights into in vitro and in situ crystallisation for X-ray and electron diffraction Keywords: Microfluidics, droplets, synchronization, sample delivery Microfluidic Tools Reducing Sample Amount in Serial Crystallography with XFELs Arizona State University, Tempe, United States of America 11:25am - 11:45am
ID: 972 / MS-42: 4 Biological and macromolecular crystallography Oral/poster MS: Machine learning in biological and structural sciences, Ultra-high resolution macromolecular crystallography and quantum biocrystallography, Novel techniques and insights into in vitro and in situ crystallisation for X-ray and electron diffraction, Solution scattering and combined techniques for biological systems, including component dynamics system, Structural biology of receptors, signaling and membrane proteins, Macromolecular machines and hybrid methods to crystallography Keywords: XFEL, serial crystallography, microED, MyD88 MyD88 TIR domain higher-order assembly interactions revealed by microcrystal electron diffraction and serial femtosecond crystallography 1Australian Research Council Centre of Excellence in Advanced Molecular Imaging, Department of Chemistry and Physics, La Trobe Institute for Molecular Science, La Trobe University, Melbourne, Victoria, Australia; 2Department of Materials and Environmental Chemistry, Stockholm University, Stockholm, Sweden; 3School of Chemistry and Molecular Biosciences, The University of Queensland, Brisbane, Queensland, Australia; 4Department of Biological Chemistry, University of California Los Angeles, Los Angeles, California, USA; 5Institute for Glycomics, Griffith University, Southport, Queensland, Australia.; 6EMBL Australia Node in Single Molecule Science, University of New South Wales, Kensington, New South Wales, Australia; 7Institute for Molecular Bioscience, The University of Queensland, Brisbane, Queensland, Australia; 8Cambridge Institute for Medical Research, University of Cambridge, Cambridge, UK.; 9Linac Coherent Light Source, SLAC National Accelerator Laboratory, Menlo Park, California, USA.; 10Australian Infectious Diseases Research Centre, The University of Queensland, Brisbane, Queensland, Australia. 11:45am - 12:05pm
ID: 1101 / MS-42: 5 Biological and macromolecular crystallography Oral/poster MS: Novel techniques and insights into in vitro and in situ crystallisation for X-ray and electron diffraction Keywords: purification, crystallisation, Sulfur-SAD, JUNGFRAU; in crystallo spectroscopy The crystallomics pipeline, a shotgun approach on native proteomes to (re)discover the unsuspected 1Swiss Light Source, Paul Scherrer Institut, Forschungsstrasse 111, Villigen-PSI, 5232, Switzerland; 2European Synchrotron Radiation Facility, 38043 Grenoble, France; 3Max-Planck-Institut für Marine Mikrobiologie, Celsiusstraße 1, 28359, Bremen, Germany; 4Structural Biology Research Center, Photon Factory, Institute of Materials Structure Science, High Energy Accelerator Research Organization, Tsukuba, 305-0801, Japan.; 5Institut de Biologie Structurale (IBS), Universite ́ Grenoble Alpes, CEA, CNRS, 71 avenue des Martyrs, Grenoble Cedex 9, 38044, France 12:05pm - 12:25pm
ID: 1020 / MS-42: 6 Biological and macromolecular crystallography Oral/poster MS: Novel techniques and insights into in vitro and in situ crystallisation for X-ray and electron diffraction Keywords: Crystallisation, crystallophore, nucleating agents, structure determination, phasing Protein crystallization assisted by the crystallophore. 1Institut de Biologie Structurale, Grenoble, France; 2Ecole Normale Supérieure, Lyon, France 12:25pm - 12:45pm
ID: 900 / MS-42: 7 Biological and macromolecular crystallography Oral/poster MS: Novel techniques and insights into in vitro and in situ crystallisation for X-ray and electron diffraction Keywords: agarose hydrogel; protein crystal nucleation, serial crystallography Protein crystallisation in agarose gel, a cheap and versatile technique 1CSIC, Granada, Spain; 2Politecnico di Torino, Torino, Italy; 3Structural Biology, Molecular Biology Institute of Barcelona, Barcelona, Spain |
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