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KN-31: The contribution of crystallography to new vaccine formulations
Time:
Friday, 20/Aug/2021:
6:10pm - 7:00pm
Session Chair: Graciela Carlota Díaz de Delgado
Location:Terrace 2A
130 2nd floor
Marcia Fantini
Presentations
Using crystallography tools to improve vaccine formulations
Márcia Carvalho de Abreu Fantini1, Cristiano Luis Pinto Oliveira1, José Luiz de Souza Lopes1, Tereza da Silva Martins2, Milena Apetito Akamatsu3, Aryene Góes Trezena4, Milene Tino de Franco4, Viviane Fongaro Botosso5, Osvaldo Augusto Brazil Esteves Sant´Anna6, Nikolay Kardjilov7, Martin Kjaerulf Rasmussen8, Heloísa Nunes Bordallo8
1University of São Paulo, Physics Institute, São Paulo - SP, Brazil; 2Chemistry Department, Federal University of São Paulo, Diadema - SP, Brazil; 3Innovation Division, Butantan Institute, São Paulo - SP, Brazil; 4Imunogenetic Laboratory, Butantan Institute, São Paulo - SP, Brazil; 5Virology Laboratory, Butantan Institute, São Paulo - SP, Brazil; 6Imunochemistry Laboratory, Butantan Institute, São Paulo - SP, Brazil; 7HZB für Materialien und Energie, Helmholtz-Zentrum Berlin, Berlin, Germany; 8Niels Bohr Institute, University of Copenhagen,
This work summarizes developments attained in oral vaccine formulations based on the encapsulation of antigens inside porous silica matrices. These vaccine vehicles protect the proteins from the harsh acidic stomach medium, allowing them to reach the Peyer´s patches, inducing immunity. Focusing on the pioneer research conducted at Butantan Institute, in Brazil, the results report the optimization of the antigens´ encapsulation yield, as well as their homogeneous distribution inside the meso and macro porous network. The characterization plus modelling of pure antigens having different dimensions and their complexes, like silica with hepatitis B virus like particles and diphtheria anatoxin, were performed by Small Angle X-ray Scattering (SAXS), X-ray Absorption Spectroscopy (XAS), X-ray Phase Contrast Tomography (XPCT) and neutron and X-ray imaging. The association of these techniques with complementary ones provided a clear picture of the proposed vaccines. Mice with variable high and low humoral responses presented significant levels of antibodies, proving the efficacy of the proposed oral immunogenic complex.
