Conference Agenda

Traditional workflow in surgical pathology involves multiple labor-intensive steps, such as tissue removal, fixation, embedding, sectioning, staining, and microscopic examination, which are time-consuming, costly, and require skilled technicians. Intraoperative tumor assessment (ITA) necessitates faster histological evaluation for real-time surgical guidance, typically using frozen section (FS) technique with hematoxylin and eosin (H&E) staining. However, an FS biopsy often comes with certain limitations, such as half-an-hour turnaround time, freezing artifacts, and potential tissue loss. To overcome these limitations, virtual H&E imaging modalities have been developed over the years aiming for rapid tissue imaging with microscopic details. However, such modalities often differ from the gold standard H&E characteristic appearance, primarily because of incorporating non-H&E contrast mechanisms, thus affecting diagnostic accuracy and applicability

In this talk, I will present True-H&E rapid fresh pathology, an innovative approach leveraging third-harmonic and two-photon signals from H and E dyes to produce optically-sectioned 2D images in fresh whole-mount tissues. Our method, invaluable for intraoperative tumor prognosis, eliminates the artifacts due to freezing, physical sectioning, and non-H&E staining, enabling pathologists to distinguish normal and tumor tissues without requiring special training., while meeting the whole-slide-imaging standard and enhancing the speed, safety, and accuracy.