1:30pm - 1:45pmID: 210
/ TOM3 S02: 1
TOM 3 BioPhotonics
Fast Adaptive Optics in optically sectioned fluorescence microscopes for functional neuroimaging
Fabrice Harms1, Mathias Mercier2, Alice Guillaume-Manca2,3, Cynthia Veilly1, Xavier Levecq1, Laurent Bourdieu3, Alexandra Fragola2
1Imagine Optic, Orsay, France; 2Institut des Sciences Moléculaires d'Orsay (ISMO), Orsay, France; 3Institut de Biologie de l'Ecole Normale Supérieure (ENS-IBENS), Paris, France
We demonstrate how a novel approach for closed-loop Adaptive Optics (AO) specifically adapted to microscopy enables straightforward integration in Light-Sheet and Multiphoton microscopes, as well as fast aberration correction. We present corresponding experimental setups as well as first demonstrations of the benefits of the correction of sample-induced aberrations in zebrafish and mouse brain tissue, with the ultimate goal to enable high-speed, high-sensitivity functional imaging at large depths.
1:45pm - 2:00pmID: 327
/ TOM3 S02: 2
TOM 3 BioPhotonics
Femtosecond laser rapid prototyping and characterization of microfluidic device for particles sorting
Annalisa Volpe1, Isabella Petruzzellis2, Francesco P. Mezzapesa3, Caterina Gaudiuso3, Roberto Osellame4, Antonio Ancona2, Rebeca Martínez Vázquez34
1POLIBA, Italy; 2UNIBA, Bari; 3IFN CNR Bari; 4IFN CNR MILANO
Rapid prototyping methods for the fabrication of polymeric labs-on-a-chip (LoC) are on the rise, as they allow high degrees of precision and flexibility. In this contest, the flexibility of ultrafast laser technology enables the rapid prototyping and high-precision micromachining of 3D LoC devices with complex microfluidic channel networks. In this paper, we describe the realization process of a microfluidic tool for fully inertial particles sorting. The microfluidic network was realized in polymethyl methacrylate (PMMA), exploiting femtosecond laser technology. The multilayer device was assembled through a facile and low-cost solvent-assisted method. In particular, we studied the particle focusing in curved inertial microfluidic channel with trapezoidal cross section. A particles focusing along the walls of the device, sensitive to particle size and flow rate, was observed based on the principle of Dean-coupled inertial migration in spiral microchannel
2:00pm - 2:15pmID: 156
/ TOM3 S02: 3
TOM 3 BioPhotonics
Photonic crystal surface mode imaging for multiplexed real-time detection of antibodies, oligonucleotides, and DNA repair proteins
Galina Nifontova1, Evgeniia Gerasimovich2, Fabrice Fleury3, Alyona Sukhanova1, Igor Nabiev1
1Laboratoire de Recherche en Nanosciences, LRN, Structure Fédérative de Recherche Cap Santé, UFR de Pharmacie, Université de Reims Champagne-Ardenne, 51100 Reims, France; 2Laboratory of Nano-Bioengineering, Moscow Engineering Physics Institute, National Research Nuclear University MEPhI, 115522 Moscow, Russia; 3DNA Repair Groupe, CNRS UMR 6286, US2B, Nantes Université, 44000 Nantes, France
Sensors based on photonic crystal (PC) surface mode imaging are promising tools for label-free drug screening and discovery, diagnostics, and analysis of ligand–receptor interactions. Imaging of PC surface modes has been demonstrated to allow simultaneous real-time detection of multiple events at the sensor surface. Here, we report the engineering of a lateral-flow microfluidic assay where PC surface mode imaging is used for multiplexed detection of biomolecular targets (antibodies, oligonucleotides, and a DNA repair protein), as well as kinetic data on their interactions obtained without additional labelling or signal amplification. Our data demonstrate the suitability of the biosensing platform designed for ultrasensitive, quick, and low-cost detection and monitoring of interactions between different biomolecules.
2:15pm - 2:30pmID: 381
/ TOM3 S02: 4
TOM 3 BioPhotonics
Quartz-Enhanced photoacoustic spectroscopy for One-Health
Pietro Patimisco
University of Bari, Italy
We report on the development of Quartz-Enhanced Photoacoustic Spectroscopy (QEPAS) technology to detect 8 different air pollutants, namely CH4, NO2, CO2, N2O, CO, NO, SO2 and NH3, with the same acoustic detection module and interchangeable laser sources, to prove the modularity of the technique as well as the adaptability to different lasers. For each gas species, the fine structure of the infrared absorption bands has been simulated by using HITRAN database. Each gas species was detected with an ultimate detection limit well below their typical natural abundance in air even with a signal integration time as low as 0.1 s.
2:30pm - 2:45pmID: 366
/ TOM3 S02: 5
TOM 3 BioPhotonics
Fabrication of Plano-convex Microlenses using Two-Photon Polymerization for Bioimaging with Non-Linear Excitation Microscopy
Behjat Sadat Kariman1, Alessandra Nardini2, Marco Grassi2, Mario Marini3, Claudio Conci2, Margaux Bouzin3, Maddalena Collini3, Manuela T. Raimondi2, Giuseppe Chirico3, Roberto Osellame4, Giulio Cerullo1, Rebeca Martínez Vázquez4
1Department of Physics, Politecnico di Milano, Milan, Italy; 2Department of Chemistry, Materials and Chemical Engineering “Giulio Natta”, Politecnico di Milano, Milan, Italy; 3Department of Physics, Università degli Studi di Milano-Bicocca, Milan, Italy; 4Institute for Photonics and Nanotechnologies (IFN), CNR, Milan, Italy
A recent challenge in bioimaging is the observation and imaging of vital, thick, and complex tissues in real time and in non-invasive mode. In the last decade, non-linear excitation microscopy showed several advantages for in-vivo imaging compared to conventional confocal techniques. Nevertheless, deep tissue imaging remains challenging, especially for thick media, due to spherical aberrations induced on focused beams by the tissue. A low numerical aperture objective lens coupled to high dioptric power microlenses, implanted in the tissue, can be beneficial for the reduction of optical aberrations. In this context, we fabricated a system of plano-convex microlenses and microscaffolds on a single chip by means of two-photon polymerization), to be used for non-linear imaging of biological specimens.
2:45pm - 3:00pmID: 224
/ TOM3 S02: 6
TOM 3 BioPhotonics
Terahertz ATR sheds light on real-time exchange kinetics occurring through plasma membrane during photodynamic therapy
Xiujun Zheng1, Blandine Lordon1, Anne-Françoise Mingotaud2, Patricia Vicendo2, Rachel Brival2, Isabelle Fourquaux3, Laure Gibot2, Guilhem Gallot1
1Ecole polytechnique, France; 2IMRCP, Université de Toulouse; 3Centre de Microscopie Electronique Appliquée à la Biologie, Université de Toulouse
THz ATR spectroscopy provides, in a single measurement, the relative number of defects per membrane surface created by oxidative stress generated during photodynamic therapy (PDT), offering early, sensitive real-time information. THz spectroscopy is therefore a complementary technique to established (biological) assays and can be applied to any topic requiring the real-time examination of short-term plasma membrane permeabilization.
|