Conference Agenda

TOM2 S02: Computational, Adaptive and Freeform Optics - focus on Illumination, AR/VR and information driven systems: Computational
Tuesday, 13/Sept/2022:
2:30pm - 4:00pm

Session Chair: Juergen Czarske, Technische Universität Dresden, Germany
Location: B120

1st floor, 70 seats

2:30pm - 3:00pm
ID: 325 / TOM2 S02: 1
TOM 2 Computational, Adaptive and Freeform Optics - focus on Illumination, AR/VR and information driven systems

Imaging beyond the limits of diffraction and aberrations with computational microscopy

Andrew Robert Harvey, Guillem Carles, Michael Handley, Daniel Olesker, Jonathan Taylor, Conall Thompson, Paul Zammit, Yongzhuang Zhou, Tomas Aidukas

School of Physics and Astronomy, University of Glasgow, United Kingdom

A common requirement for microscopy of biological samples is to image with sub-cellular resolution throughout extended volumes up to 100um thick and across a field of view that may excess 1cm^2. The fundamental limits of diffraction and the practical limits of optical aberrations means that no traditional microscopy technique can achieve these goals without resorting to mechanical scanning. We report two computational microscopy techniques that define the state of the art in 3D high-resolution imaging.

We describe (1) imaging with engineering PSFs to enable three-dimensional localisation microscopy with a spatial resolution approaching one-hundredth of a wavelength throughout a volume that is more than 15 times greater than with conventional localisation microscopy and (2) a new multi-objective Fourier ptychography that enables an arbitrary scaleable increase in the speed of acquisition of gigapixel images.

3:00pm - 3:15pm
ID: 121 / TOM2 S02: 2
TOM 2 Computational, Adaptive and Freeform Optics - focus on Illumination, AR/VR and information driven systems

Computation of aberration coefficients for plane-symmetric reflective optical systems using Lie algebraic methods

Antonio Barion1, Martijn Anthonissen1, Jan ten Thije Boonkkamp1, Wilbert IJzerman1,2

1Eindhoven University of Technology, Netherlands, The; 2Signify, Netherlands, The

The Lie algebraic method offers a systematic way to find aberration coefficients of any order for plane-symmetric reflective optical systems. The coefficients derived from the Lie method are in closed form and solely depend on the geometry of the optical system. We investigate and verify the results for a single reflector. The concatenation of multiple mirrors follows from the mathematical framework.

3:15pm - 3:30pm
ID: 320 / TOM2 S02: 3
TOM 2 Computational, Adaptive and Freeform Optics - focus on Illumination, AR/VR and information driven systems

Is trans-scleral illumination the future of retinal imaging?

Joel Terry1, Daniel Martin Geddes1, Victor Ochoa-Gutierrez1, Zhiyuan Yang2, Kenneth J. Smith2, Andy R. Harvey1

1University of Glasgow, United Kingdom; 2University College London, United Kingdom

Retinal imaging is an essential tool for monitoring eye and systemic health. Traditional approaches illuminate the retina through the pupil, requiring careful real-time alignment to separate the illumination and imaging paths and prevent the faint retinal image from being swamped by Fresnel reflections, ocular scatter and fluorescence. Somewhat surprisingly, the sclera is sufficiently transmissive to enable efficient trans-scleral illumination of the retina, which prevents overlap of the illumination and imaging light paths. We discuss how this enables and enhances three emerging retinal imaging approaches based on computational imaging.

We will discuss the opportunities for employing trans-scleral illumination for: (a) ultra-widefield aberration correction using the concepts of multi-scale, multi-camera imaging, for which transscleral illumination enables reflex-free imaging for fields of view approaching 200°; for (b) phasor-spectral-fluorescence lifetime imaging with enhanced quantification and discrimination of retinal fluorophores devoid of the lens autofluorescence that plagues conventional illumination; and for (c) spectral retinal oximetry for which reduced intraocular scatter provides improved measurement of vascular contrast and more reliable oximetry.

3:30pm - 3:45pm
ID: 337 / TOM2 S02: 4
TOM 2 Computational, Adaptive and Freeform Optics - focus on Illumination, AR/VR and information driven systems

Utilizing adaptive optics to build an inverted lattice lightsheet microscope

Lars-Christian Wittig1, Marco Pretorius1, Thomas Kalkbrenner2, Jörg Siebenmorgen2

1Carl Zeiss AG, Germany; 2Carl Zeiss Microscopy GmbH, Germany

Lightsheet microscopy has become an inevitable tool for low photodamage imaging in developmental biology. Dedicated beamshapes (lattice lightsheets) have extended this unrivalled sample preservation to live cell imaging with high spatio-temporal resolution. With the Lattice Lightsheet 7 Zeiss has made this method widely available and we want to show, that the optical configuration of this microscope is defined by only three key requirements: 1. High SNR at low photodamage, 2. Diffraction limited resolution and sectioning, 3. Consumable type sample carrier. Especially, one can conclude from these demands that an adaptive optical element is essential for the imaging path to achieve high-end performance. In order to account for tolerances of the cover glass we developed a generalised Alvarez lens which fully compensates for associated wavefront errors and thereby ensures highest imaging quality.

3:45pm - 4:00pm
ID: 392 / TOM2 S02: 5
TOM 2 Computational, Adaptive and Freeform Optics - focus on Illumination, AR/VR and information driven systems

Design and realization of a miniaturized high resolution computed tomography imaging spectrometer

Simon Amann1, Tobias Haist1, Alexander Gatto2, Markus Kamm2, Alois Herkommer1

1University of Stuttgart, Institut für Technische Optik, Germany; 2Sony Europe B.V, Stuttgart Technology Center, Germany

The computed tomography imaging spectrometer (CTIS) is a relatively unknown snapshot hyperspectral camera. It utilizes computational imaging approaches to gain the hyperspectral image from a spatio-spectral smeared sensor image. We present a strongly miniaturized system with a dimension of only 36 mm x 40.5 mm x 52.8 mm and a diagonal field of view of 29°. We achieve this using a Galilean beam expander and a combination of off-the-shelf lenses, a highly aspherical imaging system from a commercial smartphone and a 13 MP monochrome smartphone image sensor. The reconstructed hyperspectral image has a spatial resolution of 400 x 300 pixel with 39 spectral channels.