TOM 1 - Silicon Photonics and Guided-Wave Optics
TOM 2 - Computational, Adaptive and Freeform Optics
TOM 3 - Optical System Design, Tolerancing and Manufacturing
TOM 4 - Bio-Medical Optics
TOM 5 - Resonant Nanophotonics
TOM 6 - Optical Materials: crystals, thin films, organic molecules & polymers, syntheses, characterization and applications
TOM 7 - Thermal radiation and energy management
TOM 8 - Non-linear and Quantum Optics
TOM 9 - Opto-electronic Nanotechnologies and Complex Systems
TOM 10 - Frontiers in Optical Metrology
TOM 11 - Tapered optical fibers, from fundamental to applications
TOM 12 - Optofluidics
TOM 13 - Advances and Applications of Optics and Photonics
EU Project Session
Early Stage Researcher Session
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“Random illumination microscopy (RIM) : some advances and biological applications”
Simon Labouesse1, Guillaume Giroussens2, Kevin Affannoukoue2, Claire Estibal1, Renaud Poincloux3, Loïc Le Goffe2, Marc Allain2, Jérome Idier4, Anne Sentenac2, Thomas Mangeat1
1LITC Core Facility, Centre de Biologie Integrative, Université de Toulouse, CNRS, UPS, 31062 Toulouse, France; 2Institut Fresnel, Aix Marseille Université, CNRS, Centrale Marseille, Marseille, France; 3Institut de Pharmacologie et de Biologie Structurale (IPBS), Université de Toulouse, CNRS, UPS, Toulouse, France; 4LS2N, CNRS UMR 6004, 1 rue de la Noë, F44321 Nantes Cedex 3, France
Current super-resolution microscopy (SRM) methods suffer from an intrinsic complexity that might curtail their routine use in cell biology. We describe here random illumination microscopy (RIM) for live-cell imaging at super-resolutions matching that of 3D structured illumination microscopy, in a robust fashion. Based on speckled illumination and variance matchning process called AlgoRIM [1-2-3], easy to implement and user-friendly, RIM is unaffected by optical aberrations on the excitation side, linear to brightness, and compatible with multicolor live-cell imaging over extended periods of time . AlgoRIM is compatible with various RIM extensions which leads to use one single including TIRF RIM, RIM, mutliplane 3DRIM,exRIM. In the best case a resolution of 76nm is possible in TIRF RIM as well as around 120-140nm on 3D live sample until 100µm depth. The recent technological advances, allow to implement RIM on a basic microscope for a rate of 1300hz in two colors, and a lightened synchronization with the scmos detectors in comparison with the SIM technology.
 Mangeat, T., et al,Super-resolved live-cell imaging using Random Illumination Microscopy. Cell Reports Methods, 1(1), 100009.
 Labouesse.et al,2020 28th European Signal Processing Conference (EUSIPCO) (pp. 785-789). IEEE.
Phase contrast imaging to detect transparent cells in the retinal ganglion cells layer
Elena Gofas Salas1,2, Nathaniel Norberg2, Céline Louapre3, Ysoline Beigneux3, Catherine Vignal-Clermont2,4, Michel Paques2, Kate Grieve1
1Sorbonne Université, INSERM, CNRS, Institut de la Vision, 17 rue Moreau, F-75012 Paris, France; 2CHNO des Quinze-Vingts, INSERM-DGOS CIC 1423, 28 rue de Charenton, F-75012 Paris, France; 3Sorbonne Université, Paris Brain Institute - ICM, Assistance Publique Hôpitaux de Paris, Inserm, CNRS, Hôpital de la Pitié Salpêtrière, CIC neurosciences, Paris, France; 4Hôpital Fondation Rothschild, Paris, France
The eye is an optical window giving access to neural networks in a non-invasive way. It is possible to find in the retina biomarkers informing about the pathological state of other parts of the human body, and in particular of the brain. Neurodegenerative diseases could thus be diagnosed early and monitored by high-resolution imaging of the retina. However, a large part of the neurons in the retina are too transparent to be detected by existing techniques. At the Quinze-Vingts hospital, we have a unique retinal imaging platform in which ophthalmologists, neurologists and engineers participate. We implemented a technique based on scanning laser ophthalmoscopy (SLO) to capture the fine variations in refractive index between retinal cells. In this project we aimed at imaging and monitor cellular changes on transparent cells in the retinal ganglion cells layer in vivo on healthy participants and patients with neurodegenerative diseases.
Wavefront shaping using acousto-optic deflectors allows fast 3D recording of neuronal activity and transcranial imaging
Optically recording unitary neuronal activity with millisecond temporal resolution, in 3D, at large depths and in the behaving animal, is a major challenge in neuroscience. Acousto- optic deflectors (AODs) are known to be fast 2D-scanning devices. We have recently shown that they can be also used a fast beam shaping devices by synchronizing the laser pulses of low-repetition rate laser (typically a 40 kHz regenerative amplifier) with the update of the acoustic pattern in the AODs. In this configuration, the wavefront of every single laser pulse is individually patterned in phase and amplitude. We implemented this technique in a 2 photon microscope to perform (i) fast 3D serial recording at kHz rate of selected individual targets and (ii) transcranial widefield 2D-imaging using aberration and scattering corrections.