11:15 - 11:45InvitedID: 259
/ TOM4 S02: 1
TOM 4 Bio-Medical Optics
Imaging lipids in atherosclerosis – towards clinical photoacoustics
Gijs Van Soest1, Antonio Lopez Marin1, Verya Daeichin2, Sophinese Iskander-Rizk3, Nuria Slijkhuis1, Mirjam Visscher1, Ton van der Steen1,4
1Erasmus MC University Medical Ctr Rotterdam, Dept. of Cardiology, Netherlands, The; 2Kaminari Medical BV, The Netherlands; 3Delft University of Technology, 3mE, Dept. of Precision and Microsystems Engineering, Delft, The Netherlands; 4Delft University of Technology, Faculty of Applied Sciences, Dept. of Imaging Physics, Delft, The Netherlands
Atherosclerosis is a lipid-driven inflammatory disease of the arteries that is the most common precursor to myocardial infarctions and a major contributor to stroke. Staging and risk assessment based on plaque morphology has suboptimal predictive ability. We aim to create better diagnostic and interventional imaging modalities, by imaging lipids in the artery wall with photoacoustic imaging. We relate images and spectroscopic signatures to the (molecular) lipid distribution. In this talk I will review our findings and outline the developments towards practical application of photoacoustics for clinical imaging in cardiovascular medicine.
11:45 - 12:00ID: 406
/ TOM4 S02: 2
TOM 4 Bio-Medical Optics
Polarization-sensitive OCT using a fiber-scanning common-path probe
Jonas Golde1, Julia Walther1,2, Jiawen Li3,4, Robert A. McLaughlin3,4, Edmund Koch1,2
1TU Dresden, Carl Gustav Carus Faculty of Medicine, Anesthesiology and Intensive Care Medicine, Clinical Sensoring and Monitoring, 01307 Dresden, Germany; 2TU Dresden, Carl Gustav Carus Faculty of Medicine, Department of Medical Physics and Biomedical Engineering, 01307 Dresden, Germany; 3Australian Research Council Centre of Excellence for Nanoscale BioPhotonics, Adelaide Medical School, The University of Adelaide, Adelaide, SA, 5005, Australia; 4Institute for Photonics and Advanced Sensing, The University of Adelaide, Adelaide, SA, 5005, Australia
Depth-resolved retardation measurements with a single-mode fiber-based common-path probe by using polarization-sensitive optical coherence tomography are presented, utilizing the constrained polarization evolution and the mirror state phenomenon for reconstruction of the round-trip measurements.
12:00 - 12:15ID: 356
/ TOM4 S02: 3
TOM 4 Bio-Medical Optics
Determining the collagen layer thickness of the human tympanic membrane using PSOCT
Svea Steuer1, Jonas Golde1, Steffen Ossmann2, Roberta Galli1, Lars Kirsten1, Matthias Bornitz2, Marcus Neudert2, Edmund Koch1
1Clinical Sensoring and Monitoring, Anesthesiology and Intensive Care Medicine, Technische Universität Dresden, Fetscherstrasse 74, 01307 Dresden, Germany; 2Otorhinolaryngology, Carl Gustav Carus Faculty of Medicine, Technische Universität Dresden, Fetscherstrasse 74, 01307 Dresden, Germany
Normal hearing ability depends on the acoustic and mechanical properties of the tympanic membrane’s collagen layer, which is surrounded by epidermal tissue. Polarization-sensitive optical coherence tomography, a non-invasive, depth-resolving technique, utilizes tissue birefringence, e.g. of collagen fibers, as an additional imaging contrast. The tympanic membrane‘s collagen layer is accessed in 3D measurements, thereby. Calculating an estimated layer thickness distribution and comparing this to results from multiphoton microscopy based on second harmonic generation, an increased thickness in adjacent regions of the malleus and annulus is determined.
12:15 - 12:30ID: 272
/ TOM4 S02: 4
TOM 4 Bio-Medical Optics
Hyperspectral Imaging based classification of occlusal stains and carious lesions
Florian Tetschke1,2, Robin Vosahlo2, Jonas Golde1, Julia Walther1,3, Christian Hannig2, Edmund Koch1
1Technische Universität Dresden, Faculty of Medicine Carl Gustav Carus, Anesthesiology and Intensive Care Medicine, Clinical Sensoring and Monitoring, Dresden, Germany; 2Technische Universität Dresden, Faculty of Medicine Carl Gustav Carus, Policlinic of Operative and Pediatric Dentistry, Dresden, Germany; 3Technische Universität Dresden, Faculty of Medicine Carl Gustav Carus, Department of Medical Physics and Biomedical Engineering, Dresden, Germany
New diagnostic techniques are required to distinguish between stains and stained incipient lesions to enable early noninvasive treatment. This in-vitro study evaluates the performance of hyperspectral imaging (HSI) based classification for occlusal lesion detection and differentiation from stains. HSI of human teeth were analyzed by classification algorithms and validated by polarized light microscopy (PLM). A kNN algorithm yielded a strong relationship of a Matthews Correlation Coefficient of 0.77. HSI based classification of occlusal stains is highly promising as a complementary method to visuo-radiographic examination for early occlusal lesion detection.
12:30 - 12:45ID: 415
/ TOM4 S02: 5
TOM 4 Bio-Medical Optics
Molecular beacon probes for sensing and silencing survivin mRNA in cell
Ambra Giannetti1, Barbara Adinolfi1, Francesco Baldini1, Mario Pellegrino2, Giovanna Sotgiu3, Sara Tombelli1, Cosimo Trono1, Greta Varchi3
1Institute of Applied Physics "N. Carrara”, CNR, 50019 Sesto Fiorentino (FI), Italy; 2Dep. of Translat. Research and New Technol. in Medicine and Surgery, University of Pisa, 56126 Pisa, Italy; 3Institute for the Organic Synthesis and Photoreactivity, CNR, 40129 Bologna, Italy
The use of antisense oligonucleotide molecular beacons, able to generate a fluorescent signal when they hybridize with their mRNA targets, allows to conjugate the ability of sensing specific mRNA with the pharmacological silencing activity, preventing the overexpression of proteins often associated to cancer development. In this context, polymethylmethacrylate nanoparticles have been used as vehicle of an oligonucleotide molecular beacon targeting survivin mRNA in A549 human lung adenocarcinoma epithelial cells in comparison with an appropriate healthy control in in-vitro experiments.
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